Team:British Columbia/Notebook

From 2012.igem.org

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==June 8==
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Tried to make more EPI 300 cells, though no obvious growth even after hours for the second time, and decided to leave it over night to see if there are any changes.
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Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.
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- Ruichen

Revision as of 17:59, 9 June 2012

British Columbia - 2012.igem.org

June 7

In the afternoon, we (Mehul and Grace) made many more K12 competent cells, which we put into storage. (The protocol is now on the wiki.) There was also talk from Jacob and John about buying $0.50 worth of gasoline/diesel from the nearest gas station to test solvent resistance of some of our cells. I left before that happened, though. (Would have been fun to see the attendant's reaction!)

- grace

June 8

Tried to make more EPI 300 cells, though no obvious growth even after hours for the second time, and decided to leave it over night to see if there are any changes.

Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.

- Ruichen