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Team:Amsterdam
From 2012.igem.org
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| - | + | '''Multi-sensing genetic devices''' offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative '''DNA-methylation''' based '''reporter system''' in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to '''detect and store''' the presence of '''any compound''' linked to a transcriptional regulator. This system allows for '''offline monitoring''' by functioning as a '''memory module'''. Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis. Furthermore, the Cellular Logbook is able to infer the '''time of signal-onset''' or '''signal-intensity''' using the natural dilution of the registered signal’s due to cell division. In short our exciting new memory module could potentially be utilized as a platform for many groundbreaking technologies. | |
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[[File:Amsterdam_thumbs_up.gif|20px]] <font color="#8C7853">[https://igem.org/Team.cgi?id=874 <font color="#8C7853">Team registration</font>]<br\> | [[File:Amsterdam_thumbs_up.gif|20px]] <font color="#8C7853">[https://igem.org/Team.cgi?id=874 <font color="#8C7853">Team registration</font>]<br\> | ||
Revision as of 12:09, 14 September 2012
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Multi-sensing genetic devices offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative DNA-methylation based reporter system in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to detect and store the presence of any compound linked to a transcriptional regulator. This system allows for offline monitoring by functioning as a memory module. Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis. Furthermore, the Cellular Logbook is able to infer the time of signal-onset or signal-intensity using the natural dilution of the registered signal’s due to cell division. In short our exciting new memory module could potentially be utilized as a platform for many groundbreaking technologies. |
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Project description |
There are no models online yet |
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Plasmid Design Tool: Under production |
