Prokaryotic System/Secretion and Yeast Genome Integration

From 2012.igem.org

(Difference between revisions)

Latest revision as of 22:57, 27 June 2012

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Yeast System

[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1]

[http://www.google.com/url?q=http%3A%2F%2Fwww.blackwellpublishing.com%2Fgenecloning%2Fpdfs%2Fchapter7.pdf&sa=D&sntz=1&usg=AFQjCNEl8DNQGCHr4QH-Rb7zmn6oSAZm2A Yeast Vectors 2]

[http://www.google.com/url?q=http%3A%2F%2Fwww.genetics.org%2Fcontent%2F122%2F1%2F19.full.pdf&sa=D&sntz=1&usg=AFQjCNHIPhHrNJnRxDusytVTLpu59shf0Q A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae]

Yeast Integrative Plasmid Vectors (YIp)

[http://partsregistry.org/Part:BBa_K300001 GAL1 and GAL10 (galactose catabolism) selection]

[http://partsregistry.org/Part:BBa_K165061 TRP1 selection]

Prokaryotic System

Promoters

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa-J23100 (strong)]

[http://partsregistry.org/Part:BBa_J23108 BBa-J23108 (medium)]

[http://partsregistry.org/Part:BBa_J23109 BBa-J23109 (weak)]
Terminators

[http://partsregistry.org/Part:BBa_B0015 BBa-B0015]
RBS

[http://partsregistry.org/Part:BBa_B0034 Constitutive]

Secretion

[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgene%2F3990775&sa=D&sntz=1&usg=AFQjCNFRRVh0vsU3zS9AzvSGko6WIQe0Rg HlyA Gene Sequence]

[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2693460&sa=D&sntz=1&usg=AFQjCNGNhjPJSpqeHsdjbo29PuRXFmWmFA A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium]

[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F1791766&sa=D&sntz=1&usg=AFQjCNFNdr3RpmJXoTQ69ehSmrLMYMRp8Q Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain]

[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2277639&sa=D&sntz=1&usg=AFQjCNHlr8EyE98_b0wRCRqamhBzJpAw8g Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins]

Kumamolisin Info

57 kDa (beta-galactosidase: 540 kDa; [http://www.mpbio.com/detailed_info.php?family_key=02104939 source])

1701 bp

567 aa

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+{| style="color:#000000;background-color:#00FFFF;" cellpadding="3" cellspacing="1" border="2" bordercolor="#800000" width="62%" align="center"
+!align="center"|[[Team:Colorado_State|Home]]
+!align="center"|[[Team:Colorado_State/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Colorado_State Official Team Profile]
+!align="center"|[[Team:Colorado_State/Project|Project]]
+!align="center"|[[Team:Colorado_State/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Colorado_State/Modeling|Modeling]]
+!align="center"|[[Team:Colorado_State/Notebook|Notebook]]
+!align="center"|[[Team:Colorado_State/Safety|Safety]]
+!align="center"|[[Team:Colorado_State/Sponsors|Sponsors]]
+!align="center"|[[Team:Colorado_State/Attributions|Attributions]]
+|}
 '''Yeast System''' <br/>
 <li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1]