Team:Osaka/week1

August 1 (Wed)

1. Preparation of LB agar plates (Amp)
2. Restriction digests of parts (See Table 1)
3. Ligation
   • 143(A): 124+1-1G(vector)
   • 144(A): 125+1-1G(vector)
   • 145(A): 126+1-1G(vector)
   • 146(A): 127+1-1G(vector)
   • 147(A): 128+1-1G(vector)
   • 148(A): 129+1-1G(vector)
   • 149(A): 138+1-1G(vector)
   • 150(A): 139+1-1G(vector)
   • 151(A): 140+1-1G(vector)
   • 152(A): 141+1-1G(vector)
4. Transformation of ligation products into DH5α E.coli

Table 1

number	Part Name	Resistance	Description
124	<bbpart>BBa_K602015</bbpart>	C	D. radiodurans PprI+PprA expression device
125	<bbpart>BBa_K602016</bbpart>	C	D. radiodurans PprI+RecA expression device
126	<bbpart>BBa_K602017</bbpart>	C	D. radiodurans PprA+RecA expression device
127	<bbpart>BBa_K602018</bbpart>	C	D. radiodurans PprI+PprM expression device
128	<bbpart>BBa_K602019</bbpart>	C	D. radiodurans PprA+PprM expression device
129	<bbpart>BBa_K602020</bbpart>	C	D. radiodurans PprM+RecA expression device
138	<bbpart>BBa_K602005</bbpart>	C	D. radiodurans PprI expression device
139	<bbpart>BBa_K602006</bbpart>	C	D. radiodurans PprA expression device
140	<bbpart>BBa_K602007</bbpart>	C	D. radiodurans PprM expression device
141	<bbpart>BBa_K602008</bbpart>	C	D. radiodurans RecA expression device

August 2 (Thu)

1. Transfer to liquid culture:143~152
   • Some parts didn't successfully transformed

August 3 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Transformation of ligation products into DH5α E.coli again:147,148,150

August 4 (Sat)

1. Transfer to liquid culture
2. Restriction digests of 1-1G<vector> with SpeI,XbaI
3. Ligation
4. Transformation of ligation products into DH5α E.coli
5. Transformation of plasmid DNA into Rosetta E.coli:143~146,149,152

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