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From 2012.igem.org

Weeks:

June			July				August				September				October				November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert

-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI
-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+

Injection into tadpoles

Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10

Construction about a new pCS2+ with RFP insert :

(Following)
-Transformation by electroporation with T10 cells
-Plates prepared