Team:Cornell/notebook/strain list

From 2012.igem.org

Strain List

CUGEM ID	Strain Species	Strain name	Brief Description	Plasmid conferred resistances	Strain Genotype	BioBrick Insert ID	Backbone Name	Plasmid details
S0000	E. coli	DH5a		N/A		N/A	N/A	N/A
S0001	S. oneidensis	JG700		N/A	_mtrB	N/A	N/A	N/A
S0002	S. oneidensis	JG1194		N/A	_Mtr	N/A	N/A	N/A
S0003	S. oneidensis	JG1519		N/A	_Mtr/_mtrB	N/A	N/A	N/A
S0004	S. oneidensis	JG1220		kan	JG700 (S1)	N/A	?	pmtrB
S0005	S. oneidensis	JG1219		kan	JG700 (S1)	N/A	?	pmtrE
S0006	S. oneidensis	JG1527		kan	JG1519 (S3)	N/A	?	pmtrB/mtrC/mtrA
S0007	S. oneidensis	JG1531		kan	JG1519 (S3)	N/A	?	pmtrE/mtrC/mtrA
S0008	S. oneidensis	JG1537	Backbone (control)	kan	JG1519 (S3)	N/A	pBBR-BB	Empty backbone
S0009	E. coli	DH5a	Failure	amp	DH5a	N/A	pBMT-1	Gibson 1 (Failed nah operon)
S0010	E. coli	DH5a	Failure	amp	DH5a	N/A	pBMT-1	Gibson 2 (Failed nah operon)
S0011	E. coli	DH5a	Failure	amp	DH5a	N/A	pBMT-1	Gibson 3 (Failed nah operon)
S0012	E. coli	DH5a	Backbone	kan	DH5a	N/A		pBBRBB-eGFP
S0013	E. coli	DH5a	ARS Reporter	kan	DH5a	?	pBBR-BB	SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0014	E. coli	DH5a	ARS Reporter	kan	DH5a	?	pBBR-BB	SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0015	E. coli	DH5a	ARS Reporter	kan	DH5a	?	pBBR-BB	Shweta's (either SP1 or SP2?)
S0016	E. coli	DH5a	ARS Reporter	kan	DH5a	?	pBBR-BB	TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0017	E. coli	DH5a	ARS Reporter	kan	DH5a	?	pBBR-BB	TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0018	E. coli	DH5a	Backbone	cm	DH5a	BBa_J04450	pSB3C5	mRFP1 in MCS; p15a origin;
S0019	E. coli	WM3064	Conjugation strain	N/A	DAP auxotroph	N/A
S0020a	S. oneidensis	CUGEM001	ARS Reporter	kan	JG700 (S1)	?		ARS Reporter w/ RBS swap (Shweta's)
S0021	P. putida	G7	Wildtype	N/A	NAH7	N/A
S0022	E. coli	DH5a	Salicylate sensing	amp	DH5a	BBa_K228227	pSB4A5	"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results."
S0023	E. coli	DH5a	Nitrogen sensing	amp	DH5a	BBa_K216005	pSB1A2	"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide"
S0024	E. coli	DH5a	oriT	amp	DH5a	BBa_J01003	pSB1A2	"Origin of transfer, homologous to mob gene in PBRBB"
S0025	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23100	BBa_J61002	Constitutive promoter (1); mRFP downsteam; pMB1 origin;
S0026	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23101	BBa_J61002	Constitutive promoter (0.7); mRFP downsteam; pMB1 origin;
S0027	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23106	BBa_J61002	Constitutive promoter (0.4); mRFP downsteam; pMB1 origin;
S0028	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23105	BBa_J61002	Constitutive promoter (0.24); mRFP downsteam; pMB1 origin;
S0029	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23114	BBa_J61002	Constitutive promoter (0.1); mRFP downsteam; pMB1 origin;
S0030	E. coli	DH5a	Promoter	amp	DH5a	BBa_J23103	BBa_J61002	Constitutive promoter (0.01); mRFP downsteam; pMB1 origin;
S0031	E. coli	DH5a	Backbone	cm	DH5a	BBa_J04450	pSB1C3	mRFP1 in MCS; pMB1 origin;
S0032	E. coli	DH5a	nah Operon	cm	DH5a		pSB1C3	nah operon in MCS; pBM1 origin;
S0033	E. coli	DH5a	lac inducible mtrB	kan	DH5a	BBa_K098984	pSB3K3	lac indubible mtrB in MCS; p15a origin;
S0034	E. coli	DH5a	SAL (Salicylate Reporter)	kan	DH5a		pBBR-BB
S0035	E. coli	DH5a	oriT + pSB3C5	cm	DH5a		pSB3C5
S0036	E. coli	DH5a	nah Operon + p29	amp	DH5a		BBa_J61002
S0037	E. coli	WM3064	p14 RFP	kan	WM3064		pBBRBB
S0038	E. coli	WM3064	p16 RFP	kan	WM3064		pBBRBB
S0039	E. coli	WM3064	p25BB	kan	WM3064		pBBRBB
S0040	E. coli	WM3064	p27BB	kan	WM3064		pBBRBB
S0041	E. coli	WM3064	p29BB	kan	WM3064		pBBRBB
S0042	E. coli	WM3064	WM_p14	kan	WM3064		pBBRBB
S0043	E. coli	WM3064	WM_p16	kan	WM3064		pBBRBB
S0044	E. coli	WM3064	WM_SAL1	kan	WM3064		pBBRBB
S0045	E. coli	WM3064	p29_nah_oriT_p17c	cm	WM3064		pSB3C5	Constitutively expressed nah operon w/ oriT
S0046	E. coli	WM3064	SAL_RFP	kan	WM3064		pBBRBB
S0047	E. coli	WM3064	SAL2	kan	WM3064		pBBRBB
S0048	E. coli	WM3064	SAL2_RFP	kan	WM3064		pBBRBB