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From 2012.igem.org

Home Team Official Team Profile Project Parts Submitted Modeling Notebook Attributions

Contents 1 Notebook for week of June 25 to July 1 1.1 Design of converter plasmid 1.2 Design of Reporter Plasmid 1.3 Spinach Aptamer 1.4 CBB5 Caffeine Inducible Promoters 1.5 Inducible Odor

Notebook for week of June 25 to July 1

Design of converter plasmid

• Razan
  • 6/26 - Tuesday: First assembly PCR for converter and control plasmid.
  • 6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

• Peter
  • 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge

Inducible Odor