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From 2012.igem.org

Home Team Official Team Profile Project Parts Submitted Modeling Notebook Attributions

Contents 1 Notebook for week of June 18 to June 24 1.1 Design of converter plasmid 1.2 Design of Reporter Plasmid 1.3 Spinach Aptamer 1.4 CBB5 Caffeine Inducible Promoters 1.5 Inducible Odor

Notebook for week of June 18 to June 24

Design of converter plasmid

etc.

iGEM person

Design of Reporter Plasmid

• Ben
  • Prepped plasmids and made frozen stocks for IGM004 (pSB3K3-I714891) (GFP), IGM005 (pSB1AK3-K081014) (RFP), and IGM006 (pSB1A2-K081009) (LasI)
  • Prepared 5 uM stocks of BioBricks sequencing primers
  • Submitted IGM004 (pSB3K3-I714891), IGM005 (pSB1AK3-K081014), and IGM006 (pSB1A2-K081009) for sequencing
  • Transformed IGM021 (pSB1A2-J06504) (RFP) into TOP10 E.C. cells; plated on LB+Cab and picked colonies for cultures; made frozen stocks; prepped and submitted for sequencing

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

Inducible Odor