Team:Shenzhen/Notebook/YAO.Sensor

From 2012.igem.org




Yao.025-001.jpg
Journal
  • Apr. 19th. 2012

    • YAO sensor seminar：

    　　Topic: Discuss the possibility of constructing a new mitochondrial sensor.

    　　Agreement: Started investigating the involvement of Mitochondria in cancer, aging and apoptosis.

    Contributors:

    - Xiaopeng XU, Junjie BU, Chao FENG, Wenjing LI, Zhikun ZHAO, Yu CHEN, Kang KANG.

  • Apr. 24th

    • 　 　 ROS is found to involve in cancer, aging and apoptosis. Mitochondria is an important place for ROS production. So if we can sense the ROS state, it might be an useful machine for our design. Next we need to investigate the mitochondria transcription machine.

    Contributors:

    - Xiaopeng XU, Junjie BU, Chao FENG, Wenjing LI, Zhikun ZHAO.

  • May. 8th

    • 　 　 Redoxilator module built by DTU-Denmark 2009 team gives up the inspiration to construct a NAD+/NADH sensor in mitochondria.

    Contributors:

    - Xiaopeng XU, Zhikun ZHAO

  • May. 28th

    • 　 　 The basic design is engineered. The REX protein combined with an activator is expressed in mitochondria and bind to ROP to enhance the expression of GFP. Next comes the searching for mitochondrial activators.

    Contributors:

    - Xiaopeng XU, Junjie BU, Zhikun ZHAO, Wenjing LI.

  • May. 29th

    • 　 　 MTF1 is a mitochondrial transcription factor. So the design of mitochondrial sensor is complete. Next comes the manufacturing of sequences.

    Contributors:

    - Xiaopeng XU, Junjie BU, Zhikun ZHAO, Wenjing LI.

  • May. 30th

    • 　　The DNA sequences engineering of mitochondrial sensor is finished.

    Contributors:

    - Xiaopeng XU, Qiwu XU, Junjie BU.

  • Jun. 6th

    • 　　Because of the disturbance from the nucleus produced MTF1, the design might work not that efficiently. So we need to find an alternative activator or redesign our module.

    Contributors:

    - Xiaopeng XU, Junjie BU, Zhikun ZHAO, Wenjing LI.

  • Jun. 10th

    • 　　Request plasmids and strains form supporters.

    Contributors:

    - Xiaopeng XU, Qiwu XU, Chao FENG, Kang KANG.

  • May. 28th

    • 　　The strains and plasmids arrived.

    Contributors:

    - Xiaopeng XU, Junjie BU, Chao FENG, Wenjing LI, Zhikun ZHAO, Kang KANG.

  • Jul. 15th

    • 　 　 The original design is changed. REX is expressed in the nucleus and transported into mitochondria. It is used as a repressor to repress the expression of GFP in mitochondria.

    Contributors:

    - Chao FENG, Xiaopeng XU, Junjie BU, Yu CHEN, Kang KANG.

  • Jul. 18th

    • 　　Remanufacturing of the DNA sequence is finished.

    Contributors:

    - Xiaopeng XU, Qiwu XU, Chao FENG.

  • Jul. 23rd- Jul. 26th

    • 　　YAO. sensor story, pictures preparation and presentation design.

    Contributors:

    - Xiaopeng XU, Chao FENG, Junjie BU, Wenjing LI, Yu CHEN, Kang KANG.

  • Aug. 2nd - Aug. 25th

    • 　　Scripts, BioBricks and pictures preparation for Wiki

    Contributors:

    - Xiaopeng XU, Min OU, Yang DENG, Yu CHEN, Kang KANG.

  • Sep. 6th - Sep. 21st

    • 　　Modeling for YAO sensor. Preparation for documents.

    Contributors:

    - Xiaopeng XU, Xuefeng LUO.