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From 2012.igem.org

Promoters & Reporters workgroup

Digestion

Miniprep 28/06/2012

masse DNA = 2000 ng Volume final = 30 uL

DNA	Concentration (ng.uL-1)	V DNA (uL)	V SpeI (uL)	V EcoRI (uL)	V XBA I (uL)	V Buffer Orange (uL)	V Buffer sup (uL)	V water (uL)
pCS2 +	362,7	5,51	0	1	1	3	0	19,49
CFP	240,1	8,33	1	1	0	0	3	16,67
GFP	209,1	9,56	1	1	0	0	3	15,44
RFP	142,8	14,01	1	1	0	0	3	10,99
YFP	196,0	10,20	1	1	0	0	3	14,80

Incubation 3h at 37 degrees Celsius

Gel Migration

Gel at 0,08%
V tae = 30 mL
m aga = 0,24 g
V bet = 3 uL

No Band for RFP (again)

Gel Extraction

see protocol on 05/07/2012

Type	Gel weight (g)	V QG (uL)	V iso (uL)
pCS2 +	0,37	1 110	370
YFP	0,17	510	170
GFP	0,23	690	230
CFP	0,19	570	190

DNA Concentration

Type	Concentration (ng.uL-1)
pCS2 +	16,7
GFP	8,5
CFP	9,1
YFP	5,6