From 2012.igem.org
Weeks:
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July |
August |
September |
October |
November |
Week 13: 3rd September - 9th September
Monday 3rd
Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI
-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+
Tuesday 3rd
Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
Following
-Transformation by electroporation with T10 cells
-Plates prepared
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