Team:Osaka/week6

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September 2 (San)

  1. Colony check
  2. miniprep(155(3~6))
  3. Tolerance assay(MitomycinC)
  4. Colony count
  5. Restriction digests(155(3~6))
  6. Electrophoresis(155(3~6))
  7. To liquid culture(152,1-5A,5-230)
  8. Transformation(1-7A(Tet))

September 3 (Mon)

  1. miniprep(5-230,1-5A)
  2. Restriction digests(5-230,1-5A)
  3. Tolerance assey(MitomycinC)
  4. Gel extraction(5-230,1-5A)
  5. Ligation(155(1-3A->5-230))
  6. Transformation(155,1-7A)

September 4 (Tue)

  1. Tolerance assay(MitomycinC)
  2. Transformation(155,1-7A,Control)
  3. To liquid culture(WT,155,152)

September 5 (Wed)

  1. Colony count
  2. Tolerance assey(MitomycinC)
  3. Transformation(155,BBa_K518010,BBa_K518000)

September 6 (Thu)

September 7 (Fri)

September 8 (Sat)

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