Team:Cambridge/Lab book/Week 10

Monday (27/08/12)

Tuesday (28/08/12)

Wednesday (29/08/12)

Thursday (30/08/12)

PCR of Mg2+ riboswitch from genomic DNA

Gel from amplification of the riboswitch DNA. Lanes 2 + 3: with 8 codon substitution. Lanes 4 + 5: without 8 codon substitution.

• Cycle settings:

• Melting - 98 &degC - 10 seconds

• Annealing - 60 &degC - 30 seconds

• Elongation - 72 &degC - 110 seconds

• Fragments of correct size produced for all except lane 5 produced. In this lane, DNA appears to have accumulated in the well, indicating it may be genomic. In future, will use lower numbers of template cells to avoid getting so much genomic DNA.

• Products extracted and purified.

Friday (31/08/12)

Saturday (01/09/12)

Sunday (02/09/12)