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From 2012.igem.org

week 3: 14 may - 18 may

Office work

[Life science symposium]

After the great symposium in Delft, we worked further with the protein prediction program phyre2. We've tried different types of modification to the monomers to see if the monomers will drastically change. Further more Thijs and Jasper worked on the webversion of the deconstructor, a program to optimize the PCR cloning strategy. Mark searched for alternative methods to determain if VLPs are formed.

[meeting]

written by: Mark

Lab work

Making competent cells

Tuesday:

culture picked for cryo-stock. grown in 10 ml LB+kan. 10000x diluted plate was used.

Wensday:

transformation results quality: all non-diluted plates overgrown: good quality quantity:

• MACH1 333 3
• DH5X 105 0

Testing CCMV protocol

Wensday:

CCMV -80 degree Celcius storage 500 ul overnight culture 500 ul 30%glycerol stock

Thursday:

CCMV plaat

1000x from overnight culture for - 80 in 30 degree Celcius

Plated cryo 1 --> for use to check wheter the storage is good enough

Plate grows so the above protocol for cryostock can be used

Friday:

Day 2-4 protocol CCMV

5 ml disassembly buffer, 5 x 1 ml in Eppendorf tubes.