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From 2012.igem.org

week 4: 21 may - 25 may

office work

lab work

21 may

Sonified 6 x 30 seconds, output 3-4, amplitude around 20 on ice! cool as much possible, 50 seconds on ice between 30 second steps. lysate in 50 ml disassembly buffer.

Centrifuged at 15000 RPM, 3 minute 25 ml is dialised, 1st time, overnight.

22 may

Negative control for SDS Non induced BL21 CCMV grew overnight in LB+kan follow protocol CCMV from day 1, after induction up to the 4th step of day 2

sample transferred to new tube stored in iGEM -20 degree Celcius as BL21 negative - iGEM SDS

24 may

BL21 Plated and greiner tube 10 ml tube

BL21 2 plates freezer BL21 2 Greiner tubes 30 degree Celcius stove