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From 2012.igem.org

week 15: 6 august - 12 august

Office work

Lab work

New electrocompetent DH5 Alfa and Mach1 E. coli cells were prepared. Transformation efficiency was determined: DH5 Alfa: 10^8 Mach1: 10^9

TuYV

• Bricking:

Last week's minipreps were digested (EcoRI, PstI), the inserts ligated into the chloramphenicol backbone from BBa_J04450 and transformed into our electrocompetent DH5 Alfa E. coli. A PCR check gave negative results, but miniprep was performed anyway.

• Expression:

Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 E. coli. Results were unsatisfying at first, so the entire procedure was done again on saturday.

Written by: Wouter

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