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From 2012.igem.org

week 14: 30 july - 5 august

Lab work

Bricking Hepatitis B

Monday:

• Miniprepping

of 8 colonies from the transformation (Hep B + BBa_J04500)

• Digestion check

of those minipreps

Wednesday:

• Second digestion check of the minipreps (with higher amount of DNA)

the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11)

TuYV

• The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!

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