Team:UC Chile/Cyano/Labook/week5

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Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012

April 2 - April 8

Tuesday

Electrophoresis gel was done with water instead of TAE.
PCR for parts to build C2.

Wednesday

Transformation of part K325005 (Lux operon).
Gibson assembly of C2.

Thursday

Synechocystis PCC6803 culture was examined with acridine orange dye. It was axenic. Synechocystis was reinoculated in BG11 medium: 10 mL bacteria + 90 mL BG medium.
E. coli was transformed with Gibson products.

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