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From 2012.igem.org

April 2 - April 8

Tuesday

• Electrophoresis gel was done with water instead of TAE.
• PCR for parts to build C2.

Wednesday

• Transformation of part K325005 (Lux operon).
• Gibson assembly of C2.

Thursday

• Synechocystis PCC6803 culture was examined with acridine orange dye. It was axenic. Synechocystis was reinoculated in BG11 medium: 10 mL bacteria + 90 mL BG medium.
• E. coli was transformed with Gibson products.

Friday

• PCR for C1. Probably did not work.