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From 2012.igem.org

Freezer

Primers

Number	Name	Sequence	Length	Tm	%GC	Comments	Date
P1	Tir1-BB-FW	cgcttctagATGACGTACTTCCCGGAGG	19	57	58		13th july
P2	Tir1-BB-Rv	ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC	24	52	29		13th july
P3	SdMTir1-BB-Fw	AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG	65	72	43		13th july
P4	SdMTir1-BB-Rv	TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG	62	76	52		13th july
P5	Tir1 SdM v02-Fw	tgcTgtgacagaggagggac	20	60	60		25th july
P6	Tir1 SdM v02-Fw	ctagAgcagcattagtcatttgg	23	55	43		25th july
P7	pCS2+BB-Fw	cgctgcagGAACTATAGTGAGTCGTATTACG	23	51	39		13th july
P8	pCS2+BB-Rv	tgcatGAATTCGAATCGATGGGATCC	21	54	48		13th july
P9	GFP-AID-BB-Fw	gaattcgcggccgcttctagagATGGCCACAACCATGGTG	40	74	58		20th july
P10	GFP-AID-BB-Rv	tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC	51	70	43		20th july
P11	p2 Hsp70-Fw	ACTGTCGACCCCGTTTAGCAGGAAATAGCC	30	67	53	Heat Shock	20th july
P12	p2 Hsp70-Rv	ACTAAGCTTATTTGCGCTCCTTACAGTTTGC	31	63	42	Heat Shock	20th july
P13	p2 Flk-1-Fw	ACTGTCGACCTGGACTCTGGGCTAGGG	27	68	63	Vasculature	20th july
P14	p2 Flk-1-Rv	TCAAAGCTTGTTGACGTTTAGTCCAGGACAG	31	64	45	Vasculature	20th july
P15	p2 ROSA-Fw	ACTGTCGACTAGATGAAGGAGAGCC	25	61	52	Ubiquitous	20th july
P16	p2 ROSA-Rv	GATAAGCTTGGATCCCCGCAAACGCAC	27	66	56	Ubiquitous	20th july
P17	p2 CarA-Fw	ACTGTCGACGTCCCATACCAGTACAATGCAAT	32	66	47	muscles	20th july
P18	p2 CarA-Rv	AGTAAGCTTGTGCTGTGATTGAATTGGCTG	30	63	43	muscles	20th july
P19	IaaH-BB-Fw	gaattcgcggccgcttctagATGCGCGAAATGATTACGC	19	55	47		23th july
P20	IaaH-BB-Rv	ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG	25	52	28		23th july
P21	IaaM-BB-Fw	gaattcgcggccgcttctagatgtttggaccggatttccc	20	56	50		23th july
P22	IaaM-BB-Rv	ctgcagcggccgctactagtattattagtcccccagcgc	39	55/73	56/59		23th july
P23	AID-Fw	cgcttctagGGAGCTGGTGCAGGCGCT	18	64	72		25th july
P24	Sp6	ATTTAGGTGACACTATA	17	41	27	for BB detection when sequenced	NP
P25	T3	AATTAACCCTCACTAAAGGG	20	50	40	for BB detection when sequenced	NP
P26	primer_sequencing_pCS2+_Fw	CAAGTGTAGCGGTCACGCTG	20	59	60		13th july
P27	primer_sequencing_pCS2+_Rv	GCGTAATCATGGTCATAGCTG	21	52	48		13th july
P28	GFP_AID_BB_Fw_v2	TGCATgaattcgcggccgcttctagagATGGCCACAACCATGGTG	45	75	56
P29	IaaH_BB_Fw_v2	TGCATgaattcgcggccgcttctagATGCGCGAAATGATTACGC	44	74	52
P30	IaaM_BB_Fw_v2	TGCATgaattcgcggccgcttctagatgtttggaccggatttccc	45	74	53
P31	IRES_BB_F	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCATTCAagagaTCCCC	50	53/74	50/52
P32	IRES_BB_R	GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgtggccatattatcatcg	49	51/72	40/49
P33	BBa_G1005	gtttcttcctgcagcggccgctactagta	29	67	55
P34	IaaM_Kozak_BB_F	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC	60	55/76	46/52
P35	IaaH_Kozak_BB_F	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc	60	54/76	47/52

Promoteurs

Name	Sequence	Length	Species of origin	Expression domain	Restriction sites (EcoRI, XbaI, SpeI, PtsI)	Reference
p2 Xlurp	tctgtgtctccaaagatgccccagtagctccccatcttattttcactgcacatgctttgtactgctgtcacttactgagctcagggacccactcacaatatacagtacacatagaaatttcacagtataaggctgattagtaattaatacagataattactacatggaattacagaaaccagtgcaattagcatcagaatttaataatcagccctgtagcatcagcttatatgacaggccaacctcattttctgcttgataatttgcaacaactcctacgcttagcttctcaacagctgctcagagcccactgagcatgtgagagtcacagacactttccaagatggtgaccccctgtgacaggtttgaagtcctggatcattgctgctattgacaagctgaaactttaggctggtgcaataagttcagtatataaaacattgcaattctagccataataatttttagggtttagttctcctttaaggtggggccttgctaaagatgacttatttgcacatttataacatctgtcttaaaatagcatcttataactcatatacaaaaaaggggttatattgctacctacatgtcactcagcattaaaaagttccatgttccatgtttaagaggacatatttacaccgtttcaaacagtgaaatattctcatcttgctcgttggcaagaatgaggttaagtttgcagtgttgaataaaagataaatagtgattagctaaatttttcggcaggtttcattgtgaaaaagatgcccataggtgaaaaaaactgtgcgcaacaaaaaaattaattgtcgcccttcgccttcaatgcatttactgaccttttggcgaaacgagtcacattcgcccatcactaaagataaattaagtagtacacttccttcagtcttaaagtgcatcaacccaatgcaagatcatattgagctagaaggggaaggaatgccatatcatggataacacagggagataaatgtatctcctgtttgtagtagaggtcctatccatcagtaaaatatgtagacctctgcttcctagtggtaattgttgtacgcctcaacatccgtttctcaactagcaaagctttctcaaagcttattaccacctggagattaagcacctagatctagttattttccttgtgattgtgatatacagtatatgggacatcctagttggtaatttgcactacaggggaacaaaatggcatattcaggaatatatcagaaagaaaagcacatgcaaatagtgacaaacaatgcactgttaaaaaacaaaaaaagagaggcaggcctgttactctaggaaattcacctttaagagggagggagatataatttactaatatcagttaatttatttgaaagagaaaaagtagtaaaccccccactctctttaatacagtataaaaaaatattaaacagattgtgaacacaattaatgtggtgttaattcatatttgtgaattggtgtacaaacattcattgattaatcaaagtgcaagttcttcaatagtgcaatcaatgtccatgattttaagattaattaattatcttaaaaaaattattcaattagttcaatttttaaaagctgtaccctgcttgtaatctaattaactaaattcatctaataagtggaattaccgagtgtaattacagaaggtagcaattatattgaattaacaatatcaaattgaattcattaatttaaagtgctagtgcaaatataattaattagaaggtgaattaattcaatgaaagttaaaaatgtatgtccaggaccaacatctgaaaaaatgtaggaaaaggaaaataatagaggtggagaggtcgcaaaaacttctataagaggtttttctatgccctgggactccacacaatggagaagacaaacactggggactgtggtctcaaattaaccgtatctatctcattgtggagaggctaagctgtactataaaaccacagatccttcagtgtcattgaaagtaaggagaatttaaaggaagagaaaatcaaatgcggcacttgaaaggctaacagcgctggatattaattaaaagaaccaagccaattctaaaaagggtttggtagagaacagatttgttaaaatcaatttgaaagttaggagactccgccaatgcactgtaataagaaaaagtatatcagtttgcaggaaaacggagaaggcaaaactgggtggctaaaagaagtgagaaaaagaaggactagaaatgaaggggtggaaacctgagtggtgtagtaaaaagcaacaataaataaaaaaacacaacatagtgaaaatattttattgccatctgtttattaaaatctgtctattctgcacctgcaattctaaaatcattgtattctgaacagctgatattgcattgctctgttggaacttgaatggcggccctgtggctatttaagcttaattctataaacaatattagaaactcgaaaatgaacacacagaatttaccagtagaggattaaagtttattttatgtaaatgaatacaaaattcttactgtttttggttgctattggccgtttagatattttttaagcaaaacattagcaaagccctcaaagttaataattcatcggttaggccgtcctttattgatccatcaatcaggattcagttttgtgctgtcaaaaacaatgtgcgtgggaacaactttatgtggagcagggactcttcatgctctggtcagaaaatgtacaggaacttggaacagagaagaagtataaagaaaataatagcatgcatcacaatgtttctggtgtgccaatgaatagctgtgtgttacaaaatctctgtcttgtataaagacaacaccagctacagtaagttaaattctaaacacatatttgtccaatacaggggcccaattaatactgcttgtgcttttacggcacaatcaatctttaagaaaacccctcaagagtttcctaactcacgctggttttcatgctgaaaaagaaagctttgtaagttacccgacctgctgcaggactttatcccagggtgtattaataggagtttttacaataatggctcatggtaatactctgattaaactcaatagctcattgcaatactttgataaagaactgtataaaaatataacacaaagaactgttctgctgttgaacccgatgtcgtatgttgagcccaatatagagtaaaaagacagctttataacgtataattgtgtataacaaagctttgtatgatagtatacaaggcagccttataatccataatcctgtataatgaagcgttgtgcaagagaaatcatgtattatctaatatgcttatgatttctgaagcccttatcagactcttctttattataaataggggcacaatatgctattcagctaccagctagctaagataagatctctgtttcggatgtgcttgtgggaaacaagatctgttattaaccaaatcattatgagttattgactaagcctctcaatgagcttactgtttgaaagcatatataaactgggggtcaccatttggtcagttgagatctcacttgtggggttgacgcgtgccgccacaggaccttccgtgctccgtttgagggattcaatctgctgactgttccaggatctcccagacttggttgcagattgtttgctttgctcttttactcattcggtaatgtatatcttcttcattctatgtatgtaataaacctttgcttatactagctggtgtgtttgaattacttccgagagactcatagaaccacaattaaaacacagggtaacaaagtctgggcacccctgagctgacatagggcatctattgtctttgtccacagtcctgatatttttttatcaagcagtaactatgggcatgcaatttgcagtggggaaaacaagaatttggattggtgctagaactgtgtgctgctgaacaacaatgaaaactgccatcttaattctgctgaactgacacagctattttcttttttaaatatgtctattagttaaggggttaaactttaaaatgatgtagagagtaatatcccgagacaatttgctattggtttttattttttttactatttatggtatttgagctatttcgctttttattcagcagctctccagtttgcaattctggcgatctggttattaggatccaatgggtcagtgacccctgtctgaaagctgaaaagcctcctaagaagtaggcaagtcatttaaaaaacgatataaaaaaataaaataatgaagaccaagtgaaaagttgcttagaagtggccaatctatatcatacttaaagggatattgtcatgggaaaaacatttttttcaaaatgaatcagttaatagtgctgctccatcagaattctgcactgaaatccatttctcaaaagagcaaacagatttttttatattcaattttgaaatttgacatggggctagacatattgtcaatttcccagctgccccaagtcatgtgacttgtgctctgataaactttaatcactctttactgctgtactgcaagttagagtgatatcaccccctccctttttccccacagcagccaaacaaaagaacaaagggaaggtaatcagatagcagctccttaacacaagataacagctgcctggttagatataagaacaacactcaatagtaaaaacccatgtcccactgagacacattcagttacattgagaaggaaaaacagcagcctgccagaaagcatttctctctaaagtgcaggcacaagtcacatgacttggggcagctgggaaattgacaaaatgtctagccccatgtcagatttcaaaattgaatataaaaaaatctgtttgctcttttgagaaatggatttcagtgcaaaattctgctggagaagcactattaactatttgaaaaaaacatgttttctgatgacagtatccctttaaagttgacttcatggtaaaccacccctttgaaaacattacagactgtcagaaaagtatgtataaatggggaaaatatcaggaccacctaatttgagatttggtaggtatgtcactgtttgttacttttttcggttagtgaccttgcaacatttcctgtaacagcttatttcagtgtttacccttgttgatcaagggttacacaaattcccagaaatataacgtgcctttagtgattgtgcaatccatatagataaaaggggatcttgctggcagagttcacagaagagctgacttagaaacccact	5273bp	Xenopus Laevis	Myeloid cells	/	Smith et al., 2002

Glycerol Stocks

Number	Strain	Plasmid	Description	Date
P1	E.Coli DH5 aplpha	pCS2+	RFP ( BBA_J23100 )	13th july
P2	E.Coli DH5 aplpha	pCS2+	Auxine ()	13th july
P3	E.Coli DH5 aplpha	pCS2+	Tir1 GFP-Aid ()	19th july
P4	E.Coli DH5 aplpha	pCS2+	Tir1 GFP-Aid ()	19th july