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From 2012.igem.org

Freezer

Primers

Number	Name	Sequence	Length	Tm	%GC	Comments
P1	Tir1-BB-FW	cgcttctagATGACGTACTTCCCGGAGG	19	57	58
P2	Tir1-BB-Rv	ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC	24	52	29
P3	SdMTir1-BB-Fw	AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG	65	72	43
P4	SdMTir1-BB-Rv	TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG	62	76	52
P5	Tir1 SdM v02-Fw	tgcTgtgacagaggagggac	20	60	60
P6	Tir1 SdM v02-Fw	ctagAgcagcattagtcatttgg	23	55	43
P7	pCS2+BB-Fw	cgctgcagGAACTATAGTGAGTCGTATTACG	23	51	39
P8	pCS2+BB-Rv	tgcatGAATTCGAATCGATGGGATCC	21	54	48
P9	GFP-AID-BB-Fw	gaattcgcggccgcttctagagATGGCCACAACCATGGTG	40	74	58
P10	GFP-AID-BB-Rv	tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC	51	70	43
P11	p2 Hsp70-Fw	ACTGTCGACCCCGTTTAGCAGGAAATAGCC	30	67	53	Heat Shock
P12	p2 Hsp70-Rv	ACTAAGCTTATTTGCGCTCCTTACAGTTTGC	31	63	42	Heat Shock
P13	p2 Flk-1-Fw	ACTGTCGACCTGGACTCTGGGCTAGGG	27	68	63	Vasculature
P14	p2 Flk-1-Rv	TCAAAGCTTGTTGACGTTTAGTCCAGGACAG	31	64	45	Vasculature
P15	p2 ROSA-Fw	ACTGTCGACTAGATGAAGGAGAGCC	25	61	52	Ubiquitous
P16	p2 ROSA-Rv	GATAAGCTTGGATCCCCGCAAACGCAC	27	66	56	Ubiquitous
P17	p2 CarA-Fw	ACTGTCGACGTCCCATACCAGTACAATGCAAT	32	66	47	muscles
P18	p2 CarA-Rv	AGTAAGCTTGTGCTGTGATTGAATTGGCTG	30	63	43	muscles
P19	IaaH-BB-Fw	gaattcgcggccgcttctagATGCGCGAAATGATTACGC	19	55	47
P20	IaaH-BB-Rv	ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG	25	52	28
P21	IaaM-BB-Fw	gaattcgcggccgcttctagatgtttggaccggatttccc	20	56	50
P22	IaaM-BB-Rv	ctgcagcggccgctactagtattattagtcccccagcgc	18	55	56
P23	AID-Fw	cgcttctagGGAGCTGGTGCAGGCGCT	18	64	72
P24	Sp6	ATTTAGGTGACACTATA	17	41	27	for BB detection when sequenced
P25	T3	AATTAACCCTCACTAAAGGG	20	50	40	for BB detection when sequenced
P26	primer_sequencing_pCS2+_Fw	CAAGTGTAGCGGTCACGCTG	20	59	60