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From 2012.igem.org

week 3: 14 may - 18 may

Office work

[Life science symposium]

After the great symposium in Delft, we worked further with the protein prediction program phyre2. We've tried different types of modification to the monomers to see if the monomers will drastically change. Further more Thijs and Jasper worked on the webversion of the deconstructor, a program to optimize the PCR cloning strategy. Mark searched for alternative methods to determain if VLPs are formed.

[meeting]

written by: Mark

Lab work

Testing our competent cells

Tuesday:

• Culture picked from our cryo-stock and transformed with pUC19 vector by electroporation.

Wednesday:

• Transformation results

1. All non-diluted plates overgrown: good quality
2. Competence: (calculated from 10^4th diluted plates)
1. MACH1 3*10^8
2. DH5X 1*10^8

Testing CCMV protocol

Wednesday:

• Making CCMV-strain -80 degree Celcius storage

1. 500 ul overnight culture
2. 500 ul 30% glycerol stock

Thursday:

• CCMV-strain plated
• 1000x from overnight culture for - 80 in 30 degree Celcius
• Plated cryo 1 --> for use to check wheter the storage is good enough

Friday:

• Preparing samples for dialysis following dialysis protocol
• Start with dialysis

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