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Team:Uppsala University/Notebook
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Week 2Back to top |
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Tuesday 26 June
Participant: Pikkei Experiment: Re-streak from agar stabs: pSB1C3 - BBa_K322922 (cat + sacB) pSB1C3 - BBa_K322921 (sacB) BBa_K157000 - BBa_K243000 (Fok_a) BBa_K157000 - BBa_K243001 (Fok_i) Participant: Karl, Sabri, Pikkei Experiment: Take up from kit and transform: pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N pSB1A2 - PlacQ (BBa_K091111) from plate 5, well 12C Participant: Emy, Caroline Experiment: Start o/n culture: pSB1C3 - tetA pSB1C3 - CTX-M-15 pSB1C3 - ACC(6')_lb_cr pSB1C3 - SYFP2 pSB1C3 - red pSB3C5 - red pSB3T5 - red pSB1A3 - red pSB4A5 - red pSB1A2 - red pSB1K3 - J23101 pSB1K3 - red pSB3K3 - red MG1655 pSB4K1 -lacIs (N246Q) Wednesday 27 June Participant: Pikkei Experiment: Colony PCR and o/n culture 18. pSB1C3 – cat-sacB 19. pSB1C3 - sacB 20. BBa_K157000 – Fok_a 21. BBa_K157000 – Fok_i Screened two clones of each Left in PCR-machine o/n Participant: Sabri, Fredrik Experiment: Re-streak 15. pSB1A2 - Plac 17. pSB1A2 - PlacQ Participant: Sibel, Lisa, Sabri, Olle, Hampus, Caroline, Emy, Joakim, Joel, Anna, Anders) Experiment: Plasmid Prep and Glycerol Stock 4. pSB1A3 - red 5. pSB4A5 - red 12. pSB1A2 - B0034 13. pSB1K3 - J23101 14. MG1655 - pSB4K1 - lacI (N246Q) pSB1C3 - CTX-M-15 pSB1C3 - ACC(6')_lb_cr pSB1C3 - SYFP2 pSB1K3 - red pSB3K3 - red pSB1C3 - tetA 1. pSB1C3 - red 2. pSB3C5 - red 3. pSB3T5 - red Followed Plasmid Purification Protocol Participant: Hampus, Sabri Experiment: Cloning of 22. pEL3S15 - red and 13. pSB1K3 - J23101 30. pEL3S15 - J23101 Cut with X and P Participant: Caroline, Emy, Ellen, Joel Experiment: Assembly pSB3T5 - lacIs - TλT1 pSB3T5 - SYFP2 - TλT1 pSB3T5 - T5lac - B0034 Followed BioBrick Assembly Manual Thursday 28 June Participant: Joel Experiment: Gel Electrophoresis of yesterday's screening products 18.1-2 pSB1C3 – cat-sacB 19.1-2 pSB1C3 - sacB 20.1-2 BBa_K157000 – Fok_a 21.1-2 BBa_K157000 – Fok_i Result: None of the band were correct Participant: Caroline, Emy, Ellen, Joel Experiment: Transformation pSB3T5 - lacIs - TλT1 pSB3T5 - SYFP2 - TλT1 pSB3T5 - T5lac - B0034 Followed TOP10 transformation protocol Participant: Fredrik, Julia, Joakim K, Olle Experiment: Plasmid Prep and Glycerol Stock 18.1-2 pSB1C3 – cat-sacB 19.1-2 pSB1C3 - sacB 20.1-2 BBa_K157000 – Fok_a 21.1-2 BBa_K157000 – Fok_i Followed Plasmid Purification Protocol Participant: Lisa, Sabri Experiment: o/n culture .1-2 pSB1A2 - Plac .1-2 pSB1A2 - PlacQ Participant: Joakim, Anna Experiment: Make new SOB & SOC Participant: Tobias, Christoffer Experiment: Transformation pEL3S15 - J23101 Friday 29 June Participant: Julia, Hampus, Tobias Experiment: Re-streak pEL3S15 - J23101 Participant: Olle, Sabri, Lisa Experiment: Transformation 23. pSB1A1 - P1004 (cat) from registry well 7B 24. pSB2K3 - tetR from registry well 16P Participant: Sabri, Lisa, Josefin, Niklas Experiment: Assembly pSB1K3 - B0034 - SYFP2 pSB3C5 - J23101 - tet inv Participant: Caroline, Emy, Ellen, Joel, Tobias Experiment: Assembly pSB3T5 - PlacQ - ACC(6') pSB3T5 - PlacQ - CTX-M-15 pSB3T5 - PlacQ - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - TλT1 Participant: Caroline, Emy, Ellen, Joel, Tobias Experiment: Re-streak pSB3T5 - lacIs - TλT1 pSB3T5 - SYFP2 - TλT1 pSB3T5 - T5lac - B0034 Participant: Anna, Ellen Experiment: o/n culture J23116 Participant: Anna, Ellen Experiment: Plasmid Prep 15.1-2 pSB1A2 - Plac 17.1-2 pSB1A2 - PlacQ Saturday 30 June Participant: Hampus, Julia, Tobias Experiment: Screening pEL3S15 - J23101 pSB3T5 - lacIs - pSB3T5 - SYFP2 - pSB3T5 - pT5lac - B0034 Result: Bad gel Participant: Caroline, Fredrik, Emy, Hampus Experiment: o/n culture 30.1-4 pEL3S15 - J23101 31.1-4 pEL3T5 - lacIs - 32.1-4 pSB3T5 - SYFP2 - 33.1-2 pSB3T5- pT5lac - B0034 20.1 BBa_K157000 - Fok_a 21.1 BBa_K157000 - Fok_i Participant: Ellen, Emy, Caroline, Joel Experiment: Plasmid Prep J61002 - J23106 J61002 - J23116 Participant: Ellen, Emy, Caroline, Joel Experiment: Transformation pSB3T5 - PlacIq - AAC(6') pSB3T5 - PlacIq - CTX-M pSB3T5 - PlacIq - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - T.... Participant: Ellen, Emy, Caroline, Joel Experiment: Assembly pSB3T5 - J23106 - AAC(6') pSB3T5 - J23106 - CTX-M pSB3T5 - J23106 - tetA pSB3T5 - J23116 - AAC(6') pSB3T5 - J23116 - CTX-M pSB3T5 - J23116 - tetA Participant: Fredrik Experiment: Transformation 24. pSB2K3 - tet inv 39. pSB1K3 - B0034 - SYFP2 40. pSB3C5 - J23101 - tet inv Participant: Fredrik Experiment: Re-streak 16. pSB2K3 - pBAD 23. pSB1A1 - P1004 24. pSB1K3 - tet inv Sunday 1 July Participant: Hampus, Julia, Lisa Experiment: Ran new gel of yesterday's screening pEL3S15 - J23101 pSB3T5 - lacIs - pSB3T5 - SYFP2 - pSB3T5 - pT5lac - B0034 Result: Contamination Participant: Caroline, Emy Experiment: Transformation pSB3T5 - J23106 - AAC(6') pSB3T5 - J23106 - CTX-M pSB3T5 - J23106 - tetA pSB3T5 - J23116 - AAC(6') pSB3T5 - J23116 - CTX-M pSB3T5 - J23116 - tetA Participant: Caroline, Emy Experiment: Re-streak pSB3T5 - PlacIq - AAC(6') pSB3T5 - PlacIq - CTX-M pSB3T5 - PlacIq - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - T.... Participant: Emy, Caroline, Joel Experiment: o/n culture 31.1-4 pSB3T5 - lacIs - T... 32.1-4 pSB3T5 - SYFP2 - T... 33.1-2 pSB3T5 - pT5lac - B0034 24.1-4 pSB1K3 - tet inv 30.1-4 pEL3S15 - J23101 20.1 BBa_K157000 - Fok_a 21.2 BBa_K157000 - Fok_i 23.1-4 pSB1A1 - P1004 (BBa_P1004) 44. pEL3C15 (FRT) - Red 45. pEL3C15 - Red 46. pEL3A15 - Red 47. pEL3A15 (FRT) - Red 48. pEL3S15 (FRT) - Red 49. pEL3S15 - Red 50. pEL3K15 (FRT) - Red 51. pEL3K15 - Red Participant: Joel Experiment: Transformation from kit 41. pSB2K3 - Pcat (BBa_I14033) from plate 1, well 18P 42. pSB2K3 - pBAD (BBa_I14018) from plate 1, well 18N 43. pSB2K3 - Pcat (BBa_I14031) from plate 2, well 13B |
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Week 1Back to top |
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Prepared TOP10 competent cells
Prepared LB medium, TEB medium and selective agarplates |
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