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Team:Uppsala University/Notebook
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Thursday 28 June Participant: Joel Experiment: Gel Electrophoresis of yesterday's screening products 18.1-2 pSB1C3 – cat-sacB 19.1-2 pSB1C3 - sacB 20.1-2 BBa_K157000 – Fok_a 21.1-2 BBa_K157000 – Fok_i Result: None of the band were correct Participant: Caroline, Emy, Ellen, Joel Experiment: Transformation pSB3T5 - lacIs - TλT1 pSB3T5 - SYFP2 - TλT1 pSB3T5 - T5lac - B0034 Followed TOP10 transformation protocol Participant: Fredrik, Julia, Joakim K, Olle Experiment: Plasmid Prep and Glycerol Stock 18.1-2 pSB1C3 – cat-sacB 19.1-2 pSB1C3 - sacB 20.1-2 BBa_K157000 – Fok_a 21.1-2 BBa_K157000 – Fok_i Followed Plasmid Purification Protocol Participant: Lisa, Sabri Experiment: o/n culture 15.1-2 pSB1A2 - Plac 17.1-2 pSB1A2 - PlacIQ Participant: Joakim, Anna Experiment: Make new SOB & SOC Participant: Tobias, Christoffer Experiment: Transformation pEL3S15 - J23101 Friday 29 June Participant: Julia, Hampus, Tobias Experiment: Re-streak pEL3S15 - J23101 Participant: Olle, Sabri, Lisa Experiment: Transformation 23. pSB1A1 - P1004 (cat) from registry well 7B 24. pSB2K3 - tetR from registry well 16P Participant: Sabri, Lisa, Josefin, Niklas Experiment: Assembly pSB1K3 - B0034 - SYFP2 pSB3C5 - J23101 - tet inv Participant: Caroline, Emy, Ellen, Joel, Tobias Experiment: Assembly pSB3T5 - PlacQ - ACC(6') pSB3T5 - PlacQ - CTX-M-15 pSB3T5 - PlacQ - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - TλT1 Participant: Caroline, Emy, Ellen, Joel, Tobias Experiment: Re-streak pSB3T5 - lacIs - TλT1 pSB3T5 - SYFP2 - TλT1 pSB3T5 - T5lac - B0034 Participant: Anna, Ellen Experiment: o/n culture J23116 Participant: Anna, Ellen Experiment: Plasmid Prep 15.1-2 pSB1A2 - Plac 17.1-2 pSB1A2 - PlacQ Saturday 30 June Participant: Hampus, Julia, Tobias Experiment: Screening pEL3S15 - J23101 pSB3T5 - lacIs - pSB3T5 - SYFP2 - pSB3T5 - pT5lac - B0034 Result: Bad gel Participant: Caroline, Fredrik, Emy, Hampus Experiment: o/n culture 30.1-4 pEL3S15 - J23101 31.1-4 pEL3T5 - lacIs - 32.1-4 pSB3T5 - SYFP2 - 33.1-2 pSB3T5- pT5lac - B0034 20.1 BBa_K157000 - Fok_a 21.1 BBa_K157000 - Fok_i Participant: Ellen, Emy, Caroline, Joel Experiment: Plasmid Prep J61002 - J23106 J61002 - J23116 Participant: Ellen, Emy, Caroline, Joel Experiment: Transformation pSB3T5 - PlacIq - AAC(6') pSB3T5 - PlacIq - CTX-M pSB3T5 - PlacIq - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - T.... Participant: Ellen, Emy, Caroline, Joel Experiment: Assembly pSB3T5 - J23106 - AAC(6') pSB3T5 - J23106 - CTX-M pSB3T5 - J23106 - tetA pSB3T5 - J23116 - AAC(6') pSB3T5 - J23116 - CTX-M pSB3T5 - J23116 - tetA Participant: Fredrik Experiment: Transformation 24. pSB2K3 - tet inv 39. pSB1K3 - B0034 - SYFP2 40. pSB3C5 - J23101 - tet inv Participant: Fredrik Experiment: Re-streak 16. pSB2K3 - pBAD 23. pSB1A1 - P1004 24. pSB1K3 - tet inv Sunday 1 July Participant: Hampus, Julia, Lisa Experiment: Ran new gel of yesterday's screening pEL3S15 - J23101 pSB3T5 - lacIs - pSB3T5 - SYFP2 - pSB3T5 - pT5lac - B0034 Result: Contamination Participant: Caroline, Emy Experiment: Transformation pSB3T5 - J23106 - AAC(6') pSB3T5 - J23106 - CTX-M pSB3T5 - J23106 - tetA pSB3T5 - J23116 - AAC(6') pSB3T5 - J23116 - CTX-M pSB3T5 - J23116 - tetA Participant: Caroline, Emy Experiment: Re-streak pSB3T5 - PlacIq - AAC(6') pSB3T5 - PlacIq - CTX-M pSB3T5 - PlacIq - tetA pSB3K3 - Plac - B0034 - SYFP2 pSB1C3 - T.... Participant: Emy, Caroline, Joel Experiment: o/n culture 31.1-4 pSB3T5 - lacIs - T... 32.1-4 pSB3T5 - SYFP2 - T... 33.1-2 pSB3T5 - pT5lac - B0034 24.1-4 pSB1K3 - tet inv 30.1-4 pEL3S15 - J23101 20.1 BBa_K157000 - Fok_a 21.2 BBa_K157000 - Fok_i 23.1-4 pSB1A1 - P1004 (BBa_P1004) 44. pEL3C15 (FRT) - Red 45. pEL3C15 - Red 46. pEL3A15 - Red 47. pEL3A15 (FRT) - Red 48. pEL3S15 (FRT) - Red 49. pEL3S15 - Red 50. pEL3K15 (FRT) - Red 51. pEL3K15 - Red Participant: Joel Experiment: Transformation from kit 41. pSB2K3 - Pcat (BBa_I14033) from plate 1, well 18P 42. pSB2K3 - pBAD (BBa_I14018) from plate 1, well 18N 43. pSB2K3 - Pcat (BBa_I14031) from plate 2, well 13B Monday 9 July Participant: Hampus, Christofer Experiment: Screening 33. pSB1A3 - PT5lac - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Result: 33, 80 and 81 showed correct bands. Participant: Anton, Joakim, Karl Experiment: Prepare for sequencing 40. pSB3C5 - J23101 - tet inv. 64 pEL3S15 - J23101 - Spot42 Experiment: O/N 82. pSB1AK3 - Plac - B0034 12. pSB1A2 - B0034 83. pUC57 - TλT1 Experiment: Assembly 39. pSB1K3 - B0034 - SYFP2 Experiment: Cloning 88. pSB1C3 - Pcat Participant: Emy, Ellen Experiment: Assembly and transformation 109. pEL3A15 - PlacIq - AAC(6') - lacIs 110. pEL3A15 - PlacIq - CTX-M - lacIs 111. pEL3A15 - PlacIq - tet-A - lacIs Participant: Lisa, Sabri Experiment: Plasmid prep. 64. pEL3S15 - J23101 - Spot42 Participant: Julia, Hampus Experiment: Amplify SpecA-casette from pEL3S15 - J23101 - Spot42 Tuesday 10 July Participant: Joel Ås Experiment: Transform 109. pEL3A15- PlacIq - AAC(6') - lacIs 110. pEL3A15 - PlacIq - CTX-M - lacIs 111. pEL3A15 - PlacIq - tetA - lacIs Participant: Anna, Emy Experiment: Assembly and Transformation 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Participant: Anna, Emy, Karl Experiment: Streak from frozen stock 9. pSB1C3 - CTX-M Experiment: O/N from frozen stock 3. pSB3T5 - red Participant: Olle Experiment: Assembly 59. pSB3T5 - J23116 - tetA 115. pSB3T5 - J23110 - tetA Experiment: Transform 59. pSB3T5 - J23116 - tetA 91. pSb1C3 - marR 115. pSB3T5 - J23110 - tetA Participant: Arvid Experiment: Control experiment Since we've had problems growing pSB3K3-pBAD/acaC on a K-backbone, we tested some different antibiotics Result: Someone probably mixed up the sample at an early stage since it grew in ampicillin. Experiment: Plasmid prep and Frozen Stock 12. pSB1A2 - B0034 33. pSB3T5 - T5lac - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 82. pSB1AK3 - Plac - B0034 83. pUC57 - TλT1 Participant: Anton Berglund Experiment: Transformation 39. pSB1K3 - B0034 - SYFP2 88. pSB1C3 - Pcat Experiment: Prep for sequencing 33. pSB3T5 - PT5lac - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 82. pSB1AK3 - Plac - B0034 84. pSB3T5 - cat-sacB Participant: Johan Experiment: re-streak pSB1C3 - marR (BioBricked) Wednesday 11 July Participant: Anton, Olle Experiment: re-streak 39. pSB1K3 - B0034 - SYFP2 88. pSB1C3 - pcat Participant: Joel Ås Experiment: screening ImmR Pxis Participant: Hampus, Julia Experiment: Transformering pEL3S15 - J23101 - B0034 - amilCP Experiment: Re-streak 64. pEL3S15 - J23101 - Spot42 Participant: Ellen, Emy Experiment: Assembly 113. pSB3T5 - J23110 - AAC(6') 53. pSB3K3 - Plac - B0034 - SYFP2 Experiment: Cloning 52. pSB1C3 - TλT1 Thursday 12 July Participant: Hampus, Julia Experiment: Screening 64. pEL3S15 - J23101 - Spot42 Result: Clone 2 and 7 showed correct band (1500 bp). Participant: Anton Experiment: Re-streak 39. pSB1K3 - B0034 - SYFP2 Participant: Johan Experiment: Screening pSB1C3 - marR_BB10 Result: Wrong band. Participant: Fredrik Experiment: Ligation 131. pSB1C3 - ImmR 132. pSB1C3 - Pxis Participant: Olle, Karl Experiment: Make some beautiful competent cells Result: Approximate 250 competent cells in our -80 freezer. Participant: Emy, Ellen Experiment: Screening 55. pSB3T5 - J23106 - CTX-M 58. pSB3T5 - J23116 - CTX-M 59. pSB3T5 - J23116 - tetA 110. pEL3A15 - PlacQ - CTX-M - lacIs 111. pEL3A15 - PlacQ - tetA - lacIs 115. pSB3T5 - J23110 - tetA Result: All bad. Participant: Anna, Fredrik Experiement: Screening marR Experiement: Assembly 112. pSB1C3 - marR Participant: Joel Experiment: Transformation 121. pEL3A18 - red 122. pEL3C18 - red 123. pEL3K18 - red Experiment: PCR purification and digestion Pxis ImmR Participant: Lisa, Sabri Experiement: Randomized PCR on pEL3S15 - J23101 - Spot42 Friday 13 July Participant: Johan Experiement: Glycerol stock and plasmid prep. marR Participant: Karl Experiment: Restreak 121. pEL3A18 - red 122. pEL3C18 - red 123. pEL3K18 - red Participant: Anna, Fredrik Experiement: Assembly and transformation 93. pSB3C5 - J23110 - tet inv. 94. pSB3C5 - J23106 - tet inv. 95. pSB3C5 - J23101 - tet inv. 96. pSB1C3 - J23106 - B0034 97. pSB1C3 - J23110 - B0034 98. pSB1C3 - J23116 - B0034 99. pSB3T5 - B0034 - SYFP2 Participant: Lisa, Sabri Experiement: Screening 64. pEL3S15 - J23101 - Spot42 Participant: Olle, Joakim Experiment: Transformation 52. pSB1C3 - TλT1 53. pSB3T5 - Plac - B0034 - SYFP2 88. pSB1C3 - pCat 113. pSB3T5 - J23110 - AAC(6') 126. pEL3S18 - red 127. pEL3A18 - red(FRT) 128. pEL3C18 - red(FRT) 129. pEL3K18 - red(FRT) 130. pEL3S18 - red(FRT) 123. pEL3A18 - red 124. pEL3C18 - red 125. pEL3K18 - red 131. pSB1C3 - ImmR 132. pSB1C3 - Pxis Participant: Hampus, Julia, Christoffer Experiment: Screening, PCR purification, DPNI treatment specR - J23101 Result: Gave correct bands. Participant: Anna, Fredrik Experiment: Nested PCR and screening OmpF LexA Result: LexA good, OmpF has to be redone. Experiment: Nested PCR OmpF Experiment: BioBrick PCR LexA OmpF Participant: Ellen, Emy Experiment: Screening 55. pSB3T5 - J23106 - CTX-M 58. pSB3T5 - J23116 - CTX-M 59. pSB3T5 - J23116 - tetA 110. pEL3A15 - PlacQ - CTX-M - lacIs 111. pEL3A15 - PlacQ - tetA - lacIs 115. pSB3T5 - J23110 - tetA Result : 58, 59 wrong, 55 showed no band, 109, 11 and 155 were right. Experiment: O/N 109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs 111. pEL3A15 -PlacIQ - tetA - lacIs 115. pSB3T5 - J23110 - tetA Saturday 14 July Participant: Karl Experiment: Plasmid prep, glycerol stock 39. pSB1K3 - B0034 - SYFP2 109. pEL3A15 - PlacQ - AAC(6') - lacIs 111. pEL3A15 - PlacIQ - AAC(6') - lacIs 115. pSB3T5 - J23110 - tetA Experiment: O/N from frozen stock 3. pSB3T5 - red Participant: Ellen Experiment: Assembly 133. pSB1A3 - J23106 - tetA 134. pSB1A3 - J23116 - tetA 139. pSB3T5 - PT5lac - B0034 - SYFP2 55. pSB3T5 - J23116 - CTX-M 58. pSB3T5 - J23106 - CTX-M Experiment: Cloning 62. pSB1C3 - AmpR Participant: Olle Experiment: Restreak 126. pEL3S18 127. pEL3A18(FRT) 128. pEL3C18(FRT) 129. pEL3K18(FRT) 130. pEL3S18(FRT) 109. pEL3A15 - PlacIQ - AAC(6') 111. pEL3A15 - PlacIQ - tetA 115. pSB3T5 - J23110 - tetA Experiment: Transformation 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Participant: Joel Experiment: Cloning 132. pSB1C3 - Pxis Participant: Anna, Fredrik Experiment: Assembly and transformation 79. pSB1C3 - J23106 - B0034 80. pSb1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Experiment: PCR purification, digestion and ligation into pSB1C3 OmpF LexA Experiment: Restreak 93. pSB3C5 - J23110 - tet inv. 94. pSB3C5 - J23106 - tet inv. 95. pSB3C5 - J23101 - tet inv. 96. pSB3T5 - B0034 - SYFP2 Experiment: Screening OmpF OmpF (BioBricked) LexA (BioBricked) Participant: Emy Experiment: Restreak 52. pSb1C3 - TλT1 88. pSB1C3 - pCat 131. pSB1C3 - ImmR 60. pSB1A3 - B0032 - SYFP2 157. pSB1C3 - J23101 - B0034 Experiment: O/N 123 pEL3A18 - red 124. pEL3C18 - red 125. pEL3K18 - red Participant: Arvid Experiment: Assembly 101. pSB3T5 - J23110 - B0034 - SYFP2 Experiment: Cloning 140. pSB1A5 - B0034 - SYFP2 141. pSB1C5 - B0034 - SYFP2 142. pSB3C3 - B0034 - SYFP2 143. pSB3T3 - B0034 - SYFP2 144. pSB3A15 - B0034 - SYFP2 145. pSB3C15 - B0034 - SYFP2 Participant: Joakim Experiment: Assembly 53. pSB3T5 - Plac - B0034 - SYFP2 Sunday 15 July Participant: Joel Experiment: Ligate 132. pSB1C3 - T0 Participant: Ellen Experiment: Transformation 55. pSB3T5 - J23106 - CTX-M 58. pSB3T5 - J23116 - CTX-M 62. pSB1C3 - AmpR 132. pSB1C3 - Pxis 134. pSB1A3 - J23116 - tetA 139. pSB3T5 - PT5lac - B0034 - SYFP2 147. pSB1C3 - OmpF 148. pSB1C3 - LexA Participant: Arvid Experiment: Transformation 100. pSB1AK3 - B1002 101. pSB3T5 - J23110 - B0034 - SYFP2 140. pSB1A5 - B0034 - SYFP2 141. pSB1C5 - B0034 - SYFP2 142. pSB3C3 - B0034 - SYFP2 143. pSB3T3 - B0034 - SYFP2 144. pSB3A15 - B0034 - SYFP2 145. pSB3C15 - B0034 - SYFP2 Experiment: Restreak 53. pSB3T5 - Plac - B0034 - SYFP2 Participant: Anna, Fredrik Experiment: 93. pSB3C5 - J23110 - tet inv. 94. pSB3C5 - J23106 - tet inv. 95. pSB3C5 - J23101 - tet inv 96. pSB3T5 - B0034 - SYFP3 Participant: Fredrik Experiment: Assembly 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Participant: Caroline, Emy Experiment: Screening 52. pSB1C3 - TλT1 131. pSB1C3 - ImmR Experiment: O/N 123. pEL3A18 - red 124. pEL3K18 - red 131. pSB1C3 - ImmR Participant: Hampus, Olle Experiment: Mutagenesis - insertion of random nucleotides into pEL3S15 - J23101 - Spot42 Experiment: Screening 111. pEL3A15 - PlacIQ - tetA 115. pSB3T5 - J23110 - tetA Participants: Olle Experiment: Restreak 109. pEL3A15 - PlacIQ - AAC(6') 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Participant: Joel, Tobias Experiment: Assembly 133. pSB1A3 - J23106 - tetA 134. pSB1A3 - J23116 - tetA Experiment: Transformation 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 119. pEL3A18 - PlacIQ - tetA - SYFP2 120. pEL3A18 - J23106 - tetA - SYFP2 121. pEL3A18 - J23106 - CTX-M - SYFP2 122. pEL3C18 - J23106 - AAC(6') - lacIs 123. pEL3C18 - J23106 - tetA - lacIs 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 137. pEL3C18 - PlacIQ - tetA - lacIs 138. pEL3C18 - PlacIq - CTX-M - lacIs VECKA 5 Monday 16 July Participant: Sabri, Lisa Experiment: Mutagenesis - sRNA library Result: No band on gel. Participant: Caroline, Katarina Experiment: Restreak 52. TλT1 55. pSB3T5 - J23106 - CTX-M 58. pSB3T5 - J23116 - CTX-M 133. pSB1A3 - J23106 - tetA 134. pSB1A3 - J23116 - tetA Experiment: O/N 52. TλT1 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Participant: Olle, Arvid Experiment: Restreak 140. pSB1A5 - B0034 - SYFP2 143. pSB3T5 - B0034 - SYFP2 144. pEL3A15 - B0034 - SYFP2 145. pEL3C15 - B0034 - SYFP2 Participant: Anton Experiment: Assembly 101. pSB3T5 - J23110 - B0034 - SYFP2 Experiment: Prepare for sequencing 39.1,2 pSB1K3 - B0034 - SYFP2 93.2,4 pSB3C5 - J23110 - tet inv. 95.4 pSB3C5 - J23101 - tet inv. 115.2,3 pSB3T5 - J23110 - tetA 131.3,4 pSB1C3 - ImmR Participant: Karl Experiment: Restreak 147. pSB1C3 - LexA 148. pSB1C3 - OmpF Participant: Julia Experiment: O/N 96. pSB3T5 - B0034 - SYFP2 Participant: Julia, Hampus Experiment: Restreak MG1655 - SpecR - J23101 Participant: Olle Experiment: Screening 52. TλT1 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M 14. pSB4K5 - lacIs 109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs Result: All good. Participant: Joel, Joakim Experiment: Plasmid prep. 131. pSb1C3 - ImmR 115. pSB3T5 - J23110 - tetA Participant: Anna, Anders Experiment: Plasmid prep. 93. pSB3C5 - J23110 - tet inv. 95. pSb3C5 - J23101 - tet inv. 96. pSB3T5 - B0034 - SYFP2 9. pSB1C3 - CTX-M 124. pEL3C18 - red Participant: Karl Experiment: Transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 101. pSB1T5 - J23110 - B0034 - SYFP2 Participant: Julia, Hampus Experiment: Plasmid prep. and glycerol stock 123. pEL3A18 - red 125. pEL3K18 - red 126. pEL3S18 - red 127. pEL3A18(FRT) - red 128. pEL3C18(FRT) - red 129. pEL3K18(FRT) - red 130. pEL3S18(FRT) - red Participant: Arvid Experiment: Restreak 53. pSB3T5 - Plac - B0034 - SYFP2 100. pSB1AK3 - B1002 Tuesday 17 July Participant:Lisa, Sabri Experiment: Mutagenesis and screening of randomized sRNA library Result: No visible bands on gel. Participant: Hampus, Julia Experiment: Screening MG1655 - SpecR - J23101 - Cam MG1655 - SpecR - J23101 - Tet 134. pSB1A3 - J23116 - tetA 133. pSB1A3 - J23106 - tetA Result: 133, 134 correct length. Preparation of the other two was done incorrectly, no bands. Participant: Johan, Anders Experiment: Screening 52. TλT1 55. J23106 - CTX-M 14. lacIs 112. pSB1C3 - marR Result: Screening has to be redone. Participant: Caroline, Katarina Experiment: Transformation 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 119. pEL3A18 - PlacIQ - tetA - SYFP2 120. pEL3A18 - J23106 - AAC(6') - SYFP2 121. pEL3A18 - J23106 - tetA - SYFP2 133. pSB1A3 - J23106 - AAC 134. pSB1A3 - J23116 - tetA 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs 146. pSB1C3 - T0 151. pEL3C18 - J23106 - AAC(6') - lacIs 152. pEL3C18 - J23106 - tetA - lacIs Experiment: Assembly 62. pSB1C3 - AmpR 132. pSB1C3 - Pxis 139. pSB3T5 - T5lac - B0034 - SYFP2 Participant: Olle Experiment: Screening 53. pSB3K3 - Plac - B0034 - SYFP2 100. pSB1AK3 - B1002 Participant: Karl Experiment: Restreak 101. pSB3T5 - J23110 - B0034 - SYFP2 Participant: Anna Experiment: Screening 147. pSB1C3 - LexA 148. pSB1C3 - OmpF Result: OmpF might be good, LexA will be redone. Participant: Anton Experiment: Plasmid prep. 55. pSB3T5 - J23106 - CTX-M Result: Too low concentration to use. Experiment: Preparation for sequencing 52. pSB1C3 - TλT1 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Participant: Joakim Experiment: Plasmid prep. 52. pSB1C3 - TλT1 53. pSB3K3 - Plac - B0034 - SYFP2 113. pSB3T5 - J23110 - AAC(6') 114. pSB3T5 - J23110 - CTX-M Wednesday 18 July Participant: Arvid, Anton Experiment: Test if chloramphenicol can increase plasmid yield, O/N of: 140. pSB1A5 - B0034 - SYFP2 143. pSB3T5 - B0034 - SYFP2 with and without addition of chloramphenicol two hours before plasmid preparation. Result: Slight increase, less than expected. Participant: Emy, Caroline, Katarina, Joel Experiment: Screening 55. pSB3T5 - J23106 - CTX-M 58. pSB3T5 - J23116 - CTX-M 133. pSB1A3 - J23106 - tetA 134. pSB1A3 - J23116 - tetA lacIs PCR product Result: 134 was wrong, the others had clones with inserts of correct lengths. Participant: Anton Experiment: Plasmid prep. 52. pSB1C3 - TλT1 55. pSB3T5 - J23106 - CTX-M 100. pSB1AK3 - B1002 148. pSB1C3 - OmpF Participant:Lisa, Sabri Experiment: Mutagenesis PCR of randomized sRNA library Result: Failed! Participant: Julia, Hampus Experiment: Screening MG1655 - SpecR - J23101 - Cam MG1655 - SpecR - J23101 - Tet Result: Both were of the right length. Experiment: O/N 101. pSB3T5 - J23110 - B0034 - SYFP2 148. pSB1C3 - OmpF 149. MG1655 - SpecR - J23101 - Tet 150. MG1655 - SpecR - J23101 - Cam Participant: Anna Experiment: Cloning and transformation 156. pJN3C1 - AmpR Participant: Anna, Joakim Experiment: Transformation 62. pSB1C3 - AmpR 94. pSB3C5 - J23106 - tet inv. 132. pSB1C3 - Pxis 139. pSB3T5 - PT5lac - B0034 - SYFP2 147. pSB1C3 - LexA lacI (from kit) Thurusday 19 July Participant: Caroline, Katarina, Emy Experiment: Screening 53. pSB3T5 - Plac - B0034 - SYFP2 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 119. pEL3A18 - PlacIQ - tetA - SYFP2 121. pEL3A18 - J23106 - tetA - SYFP2 134. pSB1A3 - J23116 - tetA 146. pSB1C3 - T0 Experiment: O/N 53. pSB3T5 - Plac - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 119. pEL3A18 - PlacIQ - tetA - SYFP2 146. pSB1C3 - T0 Experiment: Restreak 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 Participant: Sabri, Lisa Experiment: Mutagenesis of randomized sRNA library. DMSO added to prevent primers from folding secondary structures. Participant: Joakim Experiment: Restreak 94. pSB3C5 - J23106 - tet inv. 139. pSB3T5 - Pt5lac - SYFP2 155. pSB1A3 - lacI Participant: Anna Experiment: Screening 148. pSB1C3 - OmpF 11. pSB1C3 - SYFP2 and 24. pSB2K3 - tet inv. to see if they have been mixed Experiment: Assembly 132. pSB1C3 - Pxis 156. pJN3C1 - AmpR Participant: Joel Experiment: Screening 55. pSB3T5 - J23116 - CTX-M 14. lacIs (PCR product) Result: 55 wrong, lacIs correct length. Participant: Arvid, Anton Experiment: Screening, plasmid prep. and glycerol stock 101. pSB3T5 - J23110 - B0034 - SYFP2 Experiment: Transformation 158. pSB4K5 - red Participant: Julia Experiment: PCR purification 149. MG1655 - SpecR - J23101 - Tet Participant: Anders, Johan Experiment: Screening 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 120. pEL3A18 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 146. pSB1C3 - T0 151. pEL3C18 - J23106 - AAC(6') - lacIs Result: Unknown. Friday 20 July Participant: Emy, Katarina, Caroline, Joel Experiment: Screening 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 120. pEL3A18 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 146. pSB1C3 - T0 151. pEL3C18 - J23106 - AAC(6') - lacIs Result: 116, 120, 136 good! Experiment: Assembly 55. pSB3Tt5 - J23106 - CTX-M 121. pEL3A18 - J23106 - tetA - SYFP2 134. pSB1A3 - J23116 - tetA Experiment: Screening 53. pSB3K3 - Plac - B0034 - SYFP2 58. pSB3Tt5 - J23116 - CTX-M 119. pEL3A18 - PlacIQ - tetA - SYFP2 Result: 53 and 58 had the right length. Experiment: Plasmid prep. 53. pSB3K3 - Plac - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 119. pEL3A18 - PlacIQ - tetA - SYFP2 Participant: Joakim, Anna Experiment: Screening 70. pSB3C5 - J23106 - tet inv. 139. pSB3T5 - Pt5lac - B0034 - SYFP2 147. pSB1C3 - LexA 155. pSB1A3 - lacI Result: RESULTAT RESULTAT RESULTAT RESULTAT Participant: Joel Experiment: PCR amplification of lacIs with RBS and promoter removed. Screened on gel. Result: PCR failed. Participant: Sabri, Lisa Experiment: Screening pEL3S15 - J23101 - random sRNA - Spot42 Result: 10% DMSO showed a band on the gel. New PCR with higher concentrations of DMSO will be made. Participant: Anna Experiment: Transformation 55. pSB3Tt5 - J23106 - CTX-M 115. pSB3T5 - J23110 - tetA 121. pEL3A18 - J23106 - tetA - SYFP2 134. pSB1A3 - J23116 - tetA Participant: Anders, Johan Experiment: Screening 101. pSB3T5 - J23110 - B0034 - SYFP2 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 120. pEL3A18 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 146. pSB1C3 - T0 151. pEL3C18 - J23106 - AAC(6') - lacIs Result: Gels failed. T0 seemed right. Participant: Julia Experiment: Restreak 137. pEL3C18 - PlacIQ - tetA - lacIs 138. pEL3C18 - PlacIQ - CTX-M - lacIs 152. pEL3C18 - J23106 - tetA - lacIs Experiment: Glycerol stock 149. MG1655 - SpecR - J23101 - Tet Saturday 21 July Participant: Caroline, Katarina, Emy Experiment: Screening 116. pEL3C18 - J23116 - AAC(6') - lacIs 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 120. pEL3A18 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 146. pSB1C3 - T0 151. pEL3C18 - J23106 - AAC(6') - lacIs Result: 116, 120 and 136 ok, the others were wrong. Experiment: O/N 116. pEL3C18 - J23116 - AAC(6') - lacIs 120. pEL3A18 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs Experiment: Assembly 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 Experiment: Restreak 119. pEL3A18 - PlacIQ - tetA - SYFP2 137. pEL3C18 - PlacIQ 146. pSB1C3 - T0 152. pEL3C18 - J23106 - tetA - lacIs Participant: Julia Experiment: Screening 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - J23106 - lacIs 152. pEL3C18 - PlacIQ - tetA - lacIs Result: 137 and 152 good. Experiment: Restreak 137. pEL3C18 - PlacIQ - CTX-M - lacIs 152. pEL3C18 - PlacIQ - tetA - lacIs Sunday 22 July Participant: Anna Experiment: Screening 139. pSB3T5 - PT5lac - B0034 - SYFP2 147. pSB1C3 - LexA Participant: Joel, Emy, Caroline, Katarina, Karl, Pikkei Experiment: Screen 115. pSB3T5 - J23110 - tetA 119. pEL3A18 - PlacIQ - tetA - SYFP2 Monday 23 July Participant: Tobias, Christoffer Experiment: PCR cloning of cat-sacB with λ primers for chromosomal integration Participant: Sabri, Lisa Experiment: Mutagenesis, with different concentrations of DMSO pEL3S15 - J23101 - random sRNA - Spot42 Experiment: Screening pEL3S15 - J23101 - random sRNA - Spot42 Result: Gel failed! Participant: Olle Experiment: Screening 159. pSB1C3 - pLlacO - B0034 160. pSB1C3 - T5lac - B0034 161. pSB1C3 - PrecA - B0034 - SYFP2 Result: All ok! Participant: Joel, Ellen, Karl Experiment: Phosphorylation of primers lacIs_linker_CTX_F B0034(mod)_R Experiment: Mutagenesis lacIs Experiment: Plasmid prep. 115. pSB3T5 - J23110 - tetA 119. pEL3A18 - PlacIQ - tetA - SYFP2 121. pEL3A18 - J23106 - tetA - SYFP2 132. pSB1C3 - Pxis Experiment: O/N 53. pSB3K3 - Plac - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 115. pSB3T5 - J23110 - tetA 119. pEL3A18 - PlacIQ - tetA - SYFP2 146. pSB1C3 - T0 158. pSB4K5 - red Participant: Fredrik Experiment: Plasmid prep. 55. pSB3T5 - J23106 - CTX-M 139. pSB3T5 - PT5lac - B0034 - SYFP2 147. pSB1C3 - LexA Participant: Joakim, Anna Experiment: Screening 80. pSB1C3 - J23110 - B0034 Result: OK band present on gel Participant: Julia, Hampus Experiment: Assembly 114. pSB1K3 - Pxis - B0034 -SYFP2 115. pEL3S15 - ImmR - λT1 Participant: Anton Experiment: Preparation for sequencing 53. pSB3K3 - Plac - B0034 - SYFP2 55. pSB3T5 - J23106 - CTX-M 116. pEL3C18 - J23116 - AAC(6') - lacIs 120. pEL3A18 - J23106 - AAC(6') - SYFP2 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 147. pSB1C3 - LexA 148. pSB1C3 - OmpF 149. MG1655 - SpecR - J23101 Participant: Arvid, Karl Experiment: Screening 101. PSB3T5 - J23110 - B0034 - SYFP2 Result: All clones good! Participant: Karl Experiment: Assembly 134. pSB1A3 - J23116 - tetA Experiment: Transformation 134. pSB1A3 - J23116 - tetA 162. pEL3A18 - J23110 - AAC(6') - SYFP2 163. pEL3A18 - J23110 - CTX-M - SYFP2 Experiment: Screening 60. pSB1A2 - B0032 - SYFP2 Result: Three out of six clones had the right length Tuesday 24 July Participant: Caroline, Katarina, Emy Experiment: Screening 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 Result: Both had clones with correct lengths Experiment: Assembly and transformation 166. pEL3C18 - PlacIQ - AAC(6') - lacI 167. pEL3C18 - PlacIQ - CTX-M - lacI 168. pEL3C18 - PlacIQ - tetA - lacI 169. pEL3C18 - J23106 - AAC(6') - lacI 170. pEL3C18 - J23106 - tetA - lacI Experiment: Transformation pBad AmpR 33. pSB3T5 - PT5lac - B0034 101. pSB3C5 - J23110 - B0034 - SYFP2 172. pSB3C5 - tet inv. - B0034 - SYFP2 Participant: Joel, Hampus, Julia Experiment: Plasmid prep. 53. pSB3K3 - Plac - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 119. pEL3A18 - PlacIQ - tetA - SYFP2 Result: Concentration on 58 was too low for sequencing Participant: Anton, Joakim Experiment: Preparation for sequencing 53. pSB3K3 - Plac - B0034 - SYFP2 55. pSB3T5 - J23106 - CTX-M 101. pSB3C5 - J23110 - B0034 - SYFP2 116. pEL3C18 - J23116 - AAC(6') - lacIs 120. pEL3A18 - J23106 - AAC(6') - SYFP2 132. pSB1C3 - Pxis 136. pEL3C18 - PlacIQ - AAC(6') - lacIs 139. pSB3T5 - PT5lac - B0034 - SYFP2 147. pSB1C3 - LexA 149. MG1655 - SpecR - J23101 159. pSB1C3 - pLlacO - B0034 160. pSB1C3 - T5lac - B0034 161. pSB1C3 - PrecA - B0034 - SYFP2 Participant: Sabri, Lisa Experiment: Screening, phosphorylation of primers, PCR purification, DPN1 treatment pEL3S15 - J23101 - random sRNA - Spot42 Result: 10% and 14% DMSO showed bands on the gel Participant: Fredrik Experiment: Plasmid prep. 115. pSB3T5 - J23110 - tetA 146. pSB1C3 - T0 Participant: Tobias, Christoffer Experiment: Screening cat-sacB Result: Contamination, no clear bands Participant: Johan Experiment: Assembly 33. pSB1A3 - PT5lac - B0034 102. pSB3K5 - J23110 - B0034 - SYFP2 - B1002 172. pSB3C5 - tet inv. - B0034 - SYFP2 Participant: Karl Experiment: Cloning 97. pSB3T5 - pBad 156. pJN3C1 - AmpR Participant: Olle Experiment: Glycerol stock and plasmid prep. 159. pSB1C3 - PLlacO - B0034 160. pSB1C3 - T5lac - B0034 161. pSB1C3 - PrecA - B0034 - SYFP2 Participant: Julia, Hampus Experiment: Transformation 164. pEL3S15 - Pxis - B0034 - SYFP2 165. ImmR - TλT1 Experiment: O/N 52. pSB1C3 - TλT1 Participant: Joakim, Fredrik Experiment: Phosphorylation of primers LexA(D85G)_R LexA(D85G)_F marR(mut)_R marR(mut)_F Wednesday 25 July Participant: Emy, Caroline, Katarina, Ellen Experiment: Screening 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 134. pSB1A3 - J23116 - tetA 162. pEL3A18 - J23110 - AAC(6') - SYFP2 163. pEL3A18 - J23110 - CTX-M - SYFP2 Result: 134 good, 118 and 162 have to be redone. 163 will not be continued. Participant: Hampus, Julia Experiment: PCR purification, ligation pEL3S15 - J23101 - random sRNA - Spot42 Experiment: Assembly 164. pEL3S15 - Pxis - B0034 - SYFP2 165. pSB1K3 - ImmR - TλT1 Participant: Karl Experiment: Restreak 97. pSB3T5 - pBad 156. pJN3C1 - AmpR Experiment: New cloning and transformation, in case the previous one failed 97. pSB3T5 - pBad Experiment: Transformation 14. pSB4K5 - lacIs Experiment: O/N 158. pSB4k5 - red Participant: Olle Experiment: Restreak 33. pSB3T5 - PT5lac - B0034 102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002 172. pSB3C5 - tet inv. B0034 - SYFP2 Participant: Tobias, Christoffer Experiment: PCR cloning and screening 84. pSB3T5 - cat-sacB Result: Bad gel. Participant: Anton Experiment: Preparation for sequencing 60. pSB1A3 - B0032 - SYFP2 79. pSB1C3 - J23106 - B0034 112. pSB1C3 - marR 146. pSB1C3 - T0 Participant: Julia Experiment: Plasmid prep. 52. pSb1C3 - TλT1 Participant: Arvid, Anton Experiment: Screening, preparation for sequencing 60. pSB1A3 - B0032 - SYFP2 Thursday 26 July Participant: Joel Experiment: Glycerol stock and plasmid prep. 60. pSB1A3 - B0034 - SYFP2 134. pSB1A3 - J23116 - tetA Participant: Christoffer, Tobias Experiment: PCR cloning and screening 84. pSB3T5 - cat-sacB Result: Good bands. Participant: Caroline, Emy, Katarina Experiment: Screening 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 162. pEL3A18 - J23110 - AAC(6') - SYFP2 166. pEL3C18 - PlacIQ - AAC(6') - lacI 167. pEL3C18 - PlacIQ - CTX-M - lacI 168. pEL3C18 - PlacIQ - tetA - lacI 169. pEL3C18 - J23106 - AAC(6') - lacI 170. pEL3C18 - J23106 - tetA - lacI Result: 118 and 162 had good clones. Experiment: Restreak 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 Experiment: O/N 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 162. pEL3A18 - J23110 - AAC(6') - SYFP2 Participant: Hampus, Julia Experiment: Screening 164. pEL3S15 - Pxis - B0034 - SYFP2 165. pSB1K3 - ImmR - TλT1 Result: Both had clones with the right lengths. Experiment: O/N 156. pJN3C1 - AmpR 164. pEL3S15 - Pxis - B0034 - SYFP2 165. pSB1K3 - ImmR - TλT1 Participant: Karl Experiment: Screening 97. pSB3T5 - pBad 172. pSB3C5 - tet inv. - B0034 - SYFP2 Result: 172 was good, 97 was not. Participant: Olle Experiment: Restreak 33. pSB3T5 - PT5lac - B0034 102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002 Experiment: O/N 158. pSB3K5 - red Friday 27 July Participant: Tobias, Christoffer Experiment: Assembly 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Experiment: DPN1 treatment 84. pSB3T5 - cat-sacB Participant: Caroline, Emy, Katarina, Joel Experiment: Screening and O/N 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 Result: Good! Experiment: Screening 167. pEL3C18 - PlacIQ - PlacIQ - CTX-M - lacI Result: Bad! Turns out lacI was something completely different, it had been taken from the wrong well in the kit. Experiment: Assembly 166. pEL3C18 - PlacIQ - AAC(6') - lacI 167. pEL3C18 - PlacIQ - CTX-M - lacI 168. pEL3C18 - PlacIQ - tetA - lacI 169. pEL3C18 - J23106 - AAC(6') - lacI Experiment: Streak from frozen stock 37. pSB3T5 - PlacIQ - CTX-M 38. pSB3T5 - PlacIQ - tetA 56. pSB3T5 - J23106 - tetA Participant: Ellen Experiment: Assembly 173. pEL3C18 - J23106 - CTX-M - SYFP2 174. pEL3C18 - J23106 - CTX-M - lacI Experiment: Transformation 173. pEL3C18 - J23106 - CTX-M - SYFP2 174. pEL3C18 - J23106 - CTX-M - lacI 166. pEL3C18 - PlacIQ - AAC(6') - lacI 167. pEL3C18 - PlacIQ - CTX-M - lacI 168. pEL3C18 - PlacIQ - tetA - lacI 169. pEL3C18 - J23106 - AAC(6') - lacI Participant: Olle, Karl Experiment: Screening 33. pSB3T5 - PT5lac - B0034 97. pSB3T5 - pBad 102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002 Result: All wrong. Participant: Hampus Experiment: Assembly and transformation 139. pSB3T5 - PT5lac - B0034 - SYFP2 177. pSB3T5 - AmpR - T0 Experiment: Glycerol stock and plasmid prep. 156. pJN3C1 - AmpR 164. pEL3S15 - Pxis - B0034 - SYFP2 165. pSB1K3 - ImmR - TλT1 Participant: Olle Experiment: Restreak 101. pSB3T5 - J23110 - B0034 - SYFP2 Participant: Arvid Experiment: Glycerol stock and plasmid prep. 118. pEL3A18 - PlacIQ - CTX-M - SYFP2 158. pSB4K5 - red 162. pEL3A18 - J23110 - AAC(6') - SYFP2 172. pSB3C5 - tet inv. - B0034 - SYFP2 Saturday 28 July Participant: Tobias, Christoffer Experiment: Transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Participant: Arvid, Karl Experiment: Screening 33. pSB3T5 - PT5lac - B0034 97. pSB3T5 - pBad 101. pSB3T5 - J23110 - B0034 - SYFP2 158. pSB4K5 - red 172. pSB3C5 - tet inv. - B0034 - SYFP2 Result: 33 and 97 wrong, 101 contaminated and has to be screened again, 158 and 172 seem OK. Participant: Caroline, Emy, Katarina Experiment: Phosphorylation of primers for and mutagenesis of: 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 180. pEL3C18 - J23106 - AAC(6') - linker - SYFP2 Participant: Joel Experiment: Assembly and transformation 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs 151. pEL3C18 - J23106 - AAC(6') - lacIs 182. pEL3C18 - J23106 - CTX-M - lacIs 183. pSB1C3 - Ptac Participant: Karl Experiment: O/N 37. pSB3T5 - PlacIQ - CTX-M 38. pSB3T5 - PlacIQ - tetA 56. pSB3T5 - J23106 - tetA 101. pSB3T5 - J23116 - B0034 - SYFP2 - B1002 155. pSB1A2 - lacI Participant: Fredrik Experiment: Assembly 175. pSB1K3 - PLlacO - B0034 176. pSB1K3 - PT5lacO - B0034 Participant: Ellen Experiment: Glycerol stock and plasmid prep. 33. pSB3T5 - PT5lac - B0034 117. pEL3A18 - PlacIQ - AAC(6') - SYFP2 Participant: Hampus Experiment: Restreak 139. pSB3T5 - PT5lac - B0034 - SYFP2 177. pSB3T5 - AmpR - T0 Sunday 29 July Participant: Hampus Experiment: Ligation 92. pEL3S15 - J23101 - random sRNA - Spot42 Participant: Tobias, Christoffer Experiment: Restreak 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Participant: Karl Experiment: Streak from frozen stock 60. pSB1A3 - B0032 - SYFP2 Participant: Olle Experiment: Screening 101. pSB3T5 - J23110 - B0034 - SYFP2 Result: Possibly one good clone Participant: Fredrik, Pikkei Experiment: Mutagenesis PCR 112. pSB1C3 - MarR 147. pSB1C3 - LexA 148. pSB1C3 - OmpF Result: All had clones of the correct length. Participant: Caroline, Katarina, Emy, Joel Experiment: Mutagenesis, transformation 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2 Experiment: Assembly and transformation 152. pEL3C18 - J23106 - tetA - lacIs 181. pEL3A18 - J23106 - CTX-M - SYFP2 Result: All clones of 152 were red, assembly will be redone. Experiment: Restreak 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs 151. pEL3C18 - J23106 - AAC(6') - lacIs 182. pEL3C18 - J23106 - CTX-M - lacIs 183. pSB1C3 - Ptac Participant: Hampus Experiment: Restreak 139. pSB3T5 - PT5lac - B0034 - SYFP2 177. pSB3T5 - AmpR - TT0 Participant: Ellen Experiment: Glycerol stock and plasmid prep. 37. pSB3T5 -PlacIQ - CTX-M 38. pSB3T5 - PlacIQ - tetA 56. pSB3T5 - J23106 - tetA 101. pSB3T5 - J23110 - B0034 - SYFP2 - B1002 155. pSB1A2 - lacI Experiment: Streak from frozen stock 56. pSB3T5 - J23106 - tetA Monday 30 July Participant: Julia, Hampus Experiment: Screening 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 139. pSB3T5 - PT5lac - B0034 - SYFP2 177. pSB3T5 - AmpR - TT0 183. pSB1C3 - Ptac Result: 79, 80, 81, 183 gave good bands. 139 and 177 showed the wrong bands. Experiment: O/N 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 183. pSB1C3 - Ptac Participant: Anton Experiment: Assembly and transformation 184. pEL3S15 - AmpR - T0 Participant: Tobias, Christoffer Experiment: Insertion of cat-sacB onto the chromosome of MG1655-J23101 with λ-red Participant: Caroline, Emy, Katarina Experiment: Screening 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs 151. pEL3C18 - J23106 - AAC(6') - lacIs 182. pEL3C18 - J23106 - CTX-M - lacIs Result: 151 and 182 OK, 137 and 138 failed. Experiment: O/N 56. pSB3T5 - J23106 - tetA 60. pSB1A3 - B0032 - SYFP2 151. pEL3C18 - J23106 - AAC(6') - lacIs 182. pEL3C18 - J23106 - CTX-M - lacIs Experiment: Restreak 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Olle Experiment: Phosphorylation of primers pUC_BB_F pUC_BB_R pEL8to4C15_F pEL8to4C15_R Experiment: Phusion PCR to BioBrick pUC ORI from pSB1A3 Participant: Fredrik Experiment: PCR purification, ligation and transformation 112. pSB1C3 - MarR 147. pSB1C3 - LexA 148. pSB1C3 - OmpF Experiment: Screening 175. pSB1K3 - PLlacO - B0034 Result: All clones wrong. Tuesday 31 July Participant: Arvid Experiment: Prepare agar stabs for sending to other teams pSB1A3 - amilCP pSB1C3 - amilGFP pSB1C3 - FixJ pSB1C3 - YF1 pSB1C3 - B0034 - YF1 - B0034 - FixJ pSB1C3 - PfixK2 Participant: Joakim Experiment: Restreak 184. pEL3S15 - AmpR - T0 Participant: Karl Experiment: O/N 12. pSB1A2 - B0034 Experiment: PCR purification BioBricked pUC-ORI 182. pEL3C18 - J23106 - CTX-M - lacIs Experiment: Mutagenesis PCR of pEL8C15 to pEL4C15 Experiment: Screening pUC-ORI pEL4C15 Result: Both seem OK. Participant: Emy, Caroline, Katarina, Ellen Experiment: Screening 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs 181. pEL3A18 - J23106 - CTX-M - SYFP2 Result: 137 and 138 have clones of the correct lengths. 181 was all wrong. Experiment: Assembly and transformation 152. pEL3C18 - J23106 - tetA 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Joel, Karl Experiment: Screening 14. pSB4K5 - lacIs 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6´) - linker - lacIs 180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2 Result: All seem to have good clones. Experiment: O/N 137. pEL3C18 - PlacIQ - CTX-M - lacIs 138. pEL3C18 - PlacIQ - tetA - lacIs Participant: Olle Experiment: Digestion BioBricked pUC-ORI Participant: Hampus, Julia Experiment: Screening and O/N 139. pSB3T5 - PT5lac - B0034 - SYFP2 177. pSB3T5 - AmpR - TT0 Result: Possible good clones of both. Experiment: Assembly 186. pJN3K1 - AmpR - TT0 Participant: Fredrik Experiment: Plasmid prep. 1. pSB1C3 - red 12. pSB1A2 - B0034 86. pSB1A2 - PLlacO Experiment: Assembly 175. pSB1K3 - PLlacO - B0034 Participant: Julia Experiment: Plasmid prep. 60. pSB1A3 - B0032 - SYFP2 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 183. pSB1C3 - Ptac Participant: Christoffer, Tobias Experiment: Streak MG1655 - J23101 - cat-sacB on plates with chloramphenicol and sucrose. Experiment: NotI treatment 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 183. pSB1C3 - Ptac Participant: Anton, Olle Experiment: Plasmid prep. 56. pSB3T5 - J23106 - tetA 74. pEL3C15 - PlacIQ - CTX-M - lacIs - TλT1 80. pSB1C3 - J23110 - B0034 Experiment: Preparation for sequencing 151. pEL3C18 - J23106 - AAC(6') - lacIs 182. pEL3C18 - J23106 - CTX-M - lacIs 183. pSB1C3 - Ptac Wednesday 1 August Participant: Caroline, Emy, Katarina Experiment: Screening 138. pEL3C18 - tetA - lacIs Result: One good clone! Experiment: Glycerol stock and plasmid prep. post-mutagenesis pSB4K5 - lacIs 137. pEL3C18 - PLlacO - CTX-M - lacIs 138. pEL3C18 - tetA - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 180. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 Experiment: Restreak 152. pEL3A18 - J23106 - tetA - lacIs 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2 Participant: Joel Experiment: Plasmid prep. 12. pSB1A2 - B0034 Participant: Fredrik Experiment: Screening and O/N 190. pSB1C3 - MarR(mut) 191. pSB1C3 - LexA(G85D) 192. pSB1C3 - OmpF Result: All had good clones! Experiment: Transformation 175. pSB1C3 - PLlacO - B0034 Participant: Joakim, Johan Experiment: Screening 184. pEL3S15 - AmpR - T0 Result: Contamination. Construct discontinued, different backbone will be used. Participant: Hampus Experiment: Assembly 139. Plac - B0034 - SYFP2 Participant: Julia, Hampus Experiment: Restreak 139. pSB3T5 - PT5lac - B0034 - SYFP2 Result: Success! Participant: Olle, Karl Experiment: PCR purification pUC-ORI pEL8to4C15 1. pSB1C3 - red Experiment: Ligation pEL8to4C15 pSB1C3 - pUC - red Participant: Tobias, Christoffer Experiment: Restreak MG1655 - SpecR - J23101 - cat-sacB Experiment: Screening on acrylamide gel on plasmids digested with NotI 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 183. pSB1C3 - Ptac Thursday 2 August Participant: Fredrik Experiment: Glycerol stock and plasmid prep. 190. pSB1C3 - MarR(mut) 191. pSB1C3 - LexA(G85D) 192. pSB1C3 - OmpF Participant: Joel Experiment: Plasmid prep. 12. pSB1A2 - B0034 127. pEL3A18(FRT) - red Experiment: Screening and O/N 138. pEL3C18 - PlacIQ - tetA - lacIs Result: Good! Participant: Christoffer Experiment: Screening 196. MG1655 - SpecR - J23101 - cat-sacB Result: Good! Participant: Hampus Experiment: Assembly and transformation 193. pEL3S17 - plac - B0034 - SYFP2 - AmpR - TT0 194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0 195. pSB3T5 - Pxis - B0034 - SYFP2 Experiment: Glycerol stock and plasmid prep. 186. pJN3K1 - AmpR - TT0 Participant: Karl Experiment: Restreak pSB1C3 - pUC - red pEL4C15 Participant: Joel, Caroline, Emy Experiment: Screening and O/N 152. pEL3C18 - J23106 - tetA - lacIs 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 181. pEL3A18 - J23106 - CTX-M - SYFP2 Result: Good clones of all contructs! Experiment: Restreak 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Julia Experiment: Screening 139. pSB3T5 - PT5lac - B0034 - SYFP2 Result: No clones seem to be right. Experiment: Assembly and transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Friday 3 August Participant: Karl, Arvid Experiment: O/N 187. pSB1C3 - pUC - red 188. pEL4C15 - red Participant: Julia Experiment: Assembly and transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 81. pSB1C3 - J23116 - B0034 Participant: Katarina, Caroline, Emy Experiment: Plasmid prep. 152. pEL3A18 - PlacIQ - tetA - SYFP2 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 181. pEL3A18 - J23106 - CTX-M - SYFP2 Experiment: Screening 181. pEL3A18 - J23106 - CTX-M - SYFP2 Result: All bad. Experiment: Transformation 181. pEL3A18 - J23106 - CTX-M - SYFP2 Experiment: Preparation for sequencing 119. pEL3A18 - PlacIQ - tetA - SYFP2 138. pEL3C18 - PlacIQ - tetA - lacIs 152. pEL3C18 - J23106 - tetA - lacIs 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Olle Experiment: Streak from frozen 123. pEL3A18 - red 124. pEL3C18 - red 125. pEL3K18 - red 126. pEL3S18 - red Participant: Christoffer Experiment: Glycerol stock 196. MG1655 - SpecR - J23101 - cat-sacB Experiment: O/N 198. MG1655 - SpecR - J23101 - cat-sacB (without λ-tet-plasmid) Experiment: Streak 198 on sucrose and tetracycline to make sure it works as it is supposed to Participant: Ellen Experiment: Assembly 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Hampus Experiment: Restreak 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0 195. pSB3T5 - Pxis - B0034 - SYFP2 Saturday 4 August Participant: Hampus Experiment: Screening 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0 Participant: Caroline, Katarina Experiment: Restreak 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Christoffer Experiment: Glycerol stock 198. MG1655 - SpecR - J23101 - cat-sacB (without λ-Tet-plasmid) Participant: Arvid, Karl Experiment: Screening 187. pSB1C3 - pUC - red Result: Several good clones. Experiment: Assembly 33. pSB1A3 - PT5lac - B0034 Experiment: Plasmid prep. 188. pEL4C15 - red Experiment: O/N 187. pSB1C3 - pUC - red Participant: Olle Experiment: PCR amplification AmpR AmpR(FRT) CamR CamR(FRT) SpecR SpecR(FRT) TetR TetR(FRT) KanR(FRT) Experiment: Screening AmpR AmpR(FRT) CamR CamR(FRT) SpecR SpecR(FRT) Result: All except CamR(FRT) looked good. Sunday 5 August Experiment: Glycerol stock and plasmid prep. 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Arvid Experiment: Plasmid prep. of 187. pSB1C3 - pUC - red, PCR amplification of the insert, PCR purification, vacuum drying, cloning into pEL4C15 and transformation. Experiment: Restreak 33. pSB1A3 - PT5lac - B0034 Participant: Olle Experiment: Screening tetR tetR(FRT) KanR(FRT) Result: All had good clones. Experiment: PCR purification and digestion with DpnI, SacI and SalI AmpR AmpR(FRT) CamR CamR(FRT) SpecR SpecR(FRT) TetR TetR(FRT) KanR(FRT) Participant: Caroline, Katarina Experiment: Screening and O/N 181. pEL3A18 - J23106 - CTX-M - SYFP2 Result: Two good clones Monday 6 August Participant: Hampus, Fredrik Experiment: Glycerol stock and plasmid prep. 81. pSB1C3 - J23116 - B0034 159. pSB1C3 - PLlacO - B0034 Participant: Ellen, Katarina Experiment: O/N 55. pSB3T5 - J23106 - CTX-M Experiment: Plasmid prep. 181. pEL3A18 - J23106 - CTX-M - SYFP2 34. J61002 - J23106 61. J61002 - J23110 Experiment: Preparation for sequencing 181. pEL3A18 - J23106 - CTX-M - SYFP2 Participant: Sabri, Lisa Experiment: Assembly/cloning and transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 98. pEL3K17 - PT5lac Participant: Arvid Experiment: O/N 33. pSB1A3 - PT5lac - B0034 Tuesday 7 August Participant: Olle Experiment: PCR amplification for sequencing, PCR purification 199. pEL4C15 - pUC - red Participant: Anders, Niklas, Josefin Experiment: Cloning, screening, PCR purification TetM Result: Correct length on gel Participant: Anton Experiment: Preparation for sequencing 81. pSB1C3 - OmpF(rsr) 159. pSB1C3 - PLlacO - B0034 139. pSB3T5 - PT5lac - B0034 - SYFP2 190. pSB1C3 - MarR(mut) 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Hampus, Fredrik Experiment: Screening 12. pSB1A2 - B0034 35. J61002 - J23116 81. pSB1C3 - J23116 - B0034 86. pSB1A2 - PLlacO 159. pSB1C3 - PLlacO - B0034 Result: All right. Participant: Sabri, Lisa Experiment: Assembly and transformation 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 Experiment: Restreak 98. pEL3K17 - PT5lac Wednesday 8 August Participant: Lisa, Sabri Experiment: Screening 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 Result: Expected bands at 426bp, faint bands at around 400bp were observed but disappeared before a picture was taken of the gel. New gel will be run tomorrow. Experiment: Restreak 98. pEL3K17 - PT5lac Participant: Olle Experiment: Screening 33. pSB1A3 - PT5lac -B0034 28. pSB1C3 - PT5lac (used as reference) Result: 33 is incorrect, only contains the RBS. Experiment: O/N 199. pEL4C15 - pUC - red Participant: Josefin, Niklas Experiment: Cloning pSB1C3 - tetM Thursday 9 August Participant: Anna Experiment: O/N 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 Experiment: Plasmid prep. 199. pEL4C15 - pUC - red Participant: Joakim Experiment: Assembly and transformation pSB1C3 - J23106 - B0034 Participant: Sabri Experiment: Screening 98. pEL3K17 - PT5lac Result: Has to be redone. Participant: Olle Experiment: Digestion with SacI and SalI 199. pEL4C15 - pUC - red Experiment: O/N 28. pSB1C3 - PT5lac Experiment: Assembly 101. pSB3T5 - J23110 - B0034 - SYFP2 Participant: Sabri, Joel, Katarina Experiment: Mutagenesis 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs Friday 10 August Participant: Hampus, Joel Experiment: Screening 197. pEL3S17 - red Result: Wrong bands. Experiment: PCR amplification pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Hampus Experiment: Assembly and transformation 164. pSB3T5 - Pxis - B0034 - SYFP2 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 Participant: Joakim Experiment: Restreak 79. pSB1C3 - J23106 - B0034 Participant: Sabri, Lisa Experiment: PCR purification, ligation and transformation 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs Participant: Olle Experiment: Screening 199. pEL4C15 - pUC - red (cut with SacI and SalI) Result: Good! Experiment: Assembly and transformation 230. pEL4A15 - pUC - red 231. pEL4K15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red 238. pEL4T15 - pUC - red Participant: Katarina Experiment: Cloning and transformation 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Participant: Anna, Fredrik Experiment: Assembly and transformation 202. pSB1K3 - B0034(mod) - LexA(mut) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 204. pSB1K3 - B0034(mod) - marR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(mut) Participant: Anders Experiment: Assembly and transformation 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Participant: Niklas, Josefin Experiment: Screening pSB1C3 - tetM Result: Gel failed. Saturday 11 August Participant: Olle Experiment: Plasmid prep. 154. pSB1C3 - PT5lac 240. pEL3S17 - P - R - lacIQ Experiment: Restreak 230. pEL4A15 - pUC - red 231. pEL4K15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red 238. pEL4T15 - pUC - red Participant: Hampus Experiment: Assembly 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR Experiment: Restreak 165. pSB1K3 - ImmR - TλT1 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 Participant: Anna, Karl Experiment: Assembly and transformation 202. pSB1K3 - B0034(mod) - LexA(G58D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 204. pSB1K3 - B0034(mod) - marR(mut) Experiment: Restreak 211. pSB3T5 - PLlacO - B0034 - LexA(G58D) Experiment: O/N 79. pSB1C3 - J23106 - B0034 239. pSB1A3 - J23101 - B0034 Participant: Joel, Katarina Experiment: Restreak 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Participant: Olle, Arvid Experiment: Screening pBad/AraC amplified from MG1655 chromosome Result: Good. Sunday 12 August Participant: Anders Experiment: Restreak 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Participant: Joakim Experiment: Plasmid prep. 79. pSB1A3 - J23101 - B0034 239. pSB1A3 - J23101 - B0034 Experiment: Mutagenesis (addition of B0034 to PT5lac) Participant: Anna Experiment: Restreak 202. pSB1K3 - B0034(mod) - LexA(G58D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 204. pSB1K3 - B0034(mod) - marR(mut) Experiment: Screening 211. pSB3T5 - PLlacO - B0034 - LexA(G58D) Result: No correct bands! Participant: Anders B Experiment: Screening BioBricked tetM Result: Looks good. Participant: Fredrik Experiment: Screening and O/N 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - CTX-M - linker - SYFP2 225. pEL3C18 - PlacIQ - tetA - linker - SYFP2 Result: All had good clones. Participant: Olle Experiment: Screening and O/N 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 Result: All had good clones. Experiment: Screening 230. pEL4A15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red Result: Gel failed, has to be redone. Participant: Katarina Experiment: Screening and O/N 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs Result: All had good clones. Participant: Hampus Experiment: Screening 164. pSB3T5 - Pxis - B0034 - SYFP2 223. pSB3T5 - J23116 - ImmR - TλT1 Result: 223 right. One clone of 164 might be right. Monday 13 August Participant: Karl, Arvid Experiment: Screening 101. pSB3T5 - J23110 - B0034 - SYFP2 33. pSB1C3 - PT5lac - B0034 Result: 101 good, 33 has to be screened again. Participant: Anna, Fredrik Experiment: Screening 202. pSB1K3 - B0034(mod) - LexA(G58D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 204. pSB1K3 - B0034(mod) - marR(mut) Result: All clones of 204 were good, 203 had one good clone, 202 failed. Participant: Olle Experiment: Screening 230. pEL4A15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red Result: All good! Experiment: O/N 203. pSB1K3 - B0034(mod) - OmpF(rsr) 230. pEL4A15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red 238. pEL4T5 - pUC - red Participant: Anders, Johan Experiment: Screening 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Result: Contaminated. Participant. Sabri, Lisa Experiment: Plasmid prep. 164. pSB3T5 - Pxis - B0034 - SYFP2 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Participant: Joel Experiment: Glycerol stock and plasmid prep. 241. pEL3S17-laciq - red 242. pEL3K16-laciq - red Participant: Katarina, Caroline Experiment: Plasmid prep. 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 219. pEL3C18 - J23106 - tetA - linker - lacIs Participant: Hampus Experiment: Screening 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR Result: All bad. Participant: Anton Experiment: Preparation for sequncing 164. pSB3T5 - Pxis - B0034 - SYFP2 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ - tetA - linker - SYFP2 217. pEL3A18 - J23106 - tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Tuesday 14 August Participant: Caroline, Katarina Experiment: Assembly 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs Participant: Hampus Experiment: BioBrick PCR, PCR purification and screening AmpR Result: Gave bands of the right length. Experiment: Assembly (using newly BioBricked AmpR) 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR 229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR Participant: Johan Experiment: Assembly and transformation 202. pSB1K3 - B0034 - LexA(G85D) Participant: Arvid Experiment: Screening 33. pSB1C3 - B0034 - PT5lac Result: Very good! Participant: Lisa Experiment: O/N 241. pEL3S17iq - red Participant: Anton Experiment: Preparation for sequencing 33. pSB1C3 - PT5lac B0034 79. pSB1C3 - J23106 - B0034 80. pSB1C3 - J23110 - B0034 101. pSB3T5 - J23110 - B0034 - SYFP2 125. pEL3C18 - red 127. pEL3A18(FRT) - red 128. pEL3C18(FRT) - red 129. pEL3K18(FRT) - red 182. pEL3C18 - J23106 - CTX-M - lacIs 203. pSB1K3 - B0034(mod) - ompF(rsr) 204. pSB1K3 - B0034(mod) - marR(mut) 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 224. pSB1C3 - tetM Participant: Caroline, Katarina Experiment: Plasmid prep. 230. pEL4A15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red Participant: Joel Experiment: Promoter exchange PCR (and screening) Plac -> CP6 Result: PCR failed. Participant: Sabri, Lisa Experiment: Plasmid prep. 53. pSB3T5 - Plac - B0034 - SYFP2 182. pEL3C18 - J23106 - CTX-M - lacIs 204. pSB1K3 - B0034(mod) - marR(mut) 223. pSB3T5 - J23116 - B0034 - ImmR - TλT1 236. pEL4S15(FRT) - pUC - red 238. pEL4T15 - pUC - red 241. pEL3S17iq - red 242. pEL3K16iq - red Participant: Anders Experiment: Screening 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Result: Extra bands, different PCR protocol will be used. Participant: Joakim Experiment: Cloning and transformation pEL3S17 - Plac - b0034 - SYFP2 Wednesday 15 August Participant: Olle Experiment: Preparation for sequencing 230. pEL4A15 - pUC - red 232. pEL4S15 - pUC - red 233. pEL4A15(FRT) - pUC - red 234. pEL4C15(FRT) - pUC - red 235. pEL4K15(FRT) - pUC - red 236. pEL4S15(FRT) - pUC - red 237. pEL4T15(FRT) - pUC - red 238. pEL4T15 - pUC - red Experiment: Digestion with E+P pBad Participant: Joakim Experiment: Assembly 206. pSB3T5 - J23101 - B0034 208. pSB3T5 - J23101 - B0034 - marR(mut) Experiment: Transformation 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) Participant: Anton Experiment: Restreak 246. pEL3K18 - J23106 - B0034 - TetM 247. pEL3K18 - J23110 - B0034 - TetM pEL3S17 - R - lacIQ Participant: Anders Experiment: Screening 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Result: Gel failed. Participant: Sabri Experiment: Transformation 98. pEL3K17 - PT5lac 228. pEL3S18 - Plac - B0034 - SYFP2 - AmpR 229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs Participant: Caroline, Katarina Experiment: Plasmid prep. 33. pSB1C3 - PT5lac - B0034 101. pSB3T5 - J23110 - B0034 - SYFP2 Participant: Arvid Experiment: O/N from frozen stock 123. pEL3A18 - red 124. pEL3C15 - red 125. pEL3K18 - red 126. pEL3S18 - red 127. pEL3A18(FRT) - red 128. pEL3C18(FRT) - red 129. pEL3K18(FRT) - red 130. pEL3S18(FRT) - red Participant: Fredrik Experiment: Screening and O/N 202. pSB1K3 - B0034(mod) - LexA(G85D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) Result: Both had good clones. Participant: Anna, Fredrik Experiment: Plasmid prep. 202. pSB1K3 - B0034(mod) - LexA(G85D) 203. pSB1K3 - B0034(mod) - OmpF Thursday 16 August Participant: Caroline, Katarina Experiment: Plasmid prep. 123. pEL3A18 - red 124. pEL3C15 - red 125. pEL3K18 - red 126. pEL3S18 - red 128. pEL3C18(FRT) - red 129. pEL3K18(FRT) - red 130. pEL3S18(FRT) - red Participant: Fredrik, Anna, Joakim Experiment: O/N 252. MG1655 (Acceptor Cipro.R) 253. R1drd19 - C.R 254. R46 - T.R 255. pSa - K.R 256. N3T - T.R Participant: Joakim Experiment: Transformation 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - marR(mut) Experiment: Cloning 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16 - PT5lac - B0034 Experiment: Restreak 245. pEL3S17 - Plac - B0034 - SYFP2 Participant: Joel Experiment: Digest and screening 60. pSB1A3 - B0032 - SYFP2 Result: No good clones. Experiment: Mutagenesis PCR, screening, PCR purification, DPNI 260. pEL3C18 - J23106 - CTX-M(5'UTR) - lacIs Result: Good! Participant: Arvid Experiment: Assembly 102. pSB3K5 - J23110 - B0034 - SYFP2 B1002 Experiment: PCR for insertion of lacIq pEL4x15 Friday 17 August Participant: Anna, Fredrik, Joakim, Karl Experiment: Test of conjugation 253. R1drd19 254. R46 255. pSA 256. N3T Participant: Caroline, Katarina Experiment: Screening 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs Result: One clone of 250 was right, the others were all wrong. Experiment: PCR purification 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Experiment: Transformation 60. pSB1A3 - B0032 - SYFP2 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Participant: Joakim Experiment: Restreak 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - marR(mut) Experiment: Transformation 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16 - PT5lac - B0034 Participant: Sabri, Lisa Experiment: Making competent MG1655 cells Experiment: Transformation 79. pSB1C3 - J23106 - B0034 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655) Participant: Arvid Experiment: Transformation 102. pSB3C5 - J23110 - B0034 - SYFP2 Participant: Hampus Experiment: Screening and restreak 228. pEL3X17 - Plac - B0034 - SYFP2 - AmpR 229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR Experiment: Assembly 79. pSB3T5 - J23106 - B0034 164. pSB3T5 - Pxis - B0034 - SYFP2 Result: The parts 164 was assembled from turned out to be wrong. Saturday 18 August Participant: Joakim Experiment: Restreak 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16 - PT5lac - B0034 Experiment: Screening 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16 - PT5lac - B0034 Result: Screening has to be redone. Participant: Hampus Experiment: Transformation 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655) Experiment: Restreak 79. pSB3T5 - J23106 - B0034 Participant: Hampus, Karl Experiment: Screening 87. pSB1C3 - pBad 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR Result: 87 had no good clones, 228 has to be screened again due to contamination. Participant: Karl Experiment: O/N 87. pSB1C3 - pBad pGEM - eforRed pGEM - aeBlue pGEM - cjBlue Participant: Anna Experiment: Screening and O/N 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - marR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) Participant: Katarina, Caroline, Emy Experiment: Screening 250. pEL3C18 - J23116 - CTX-M - lacIs 257. pEL4C15 - J23110 AAC(6') - linker - SYFP2 258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2 259. pEL4C15 - J23106 - CTX-M - linker - SYFP2 Result: All had good clones. Experiment: O/N 257. pEL4C15 - J23110 AAC(6') - linker - SYFP2 258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2 259. pEL4C15 - J23106 - CTX-M - linker - SYFP2 Experiment: Glycerol stock and plasmid prep. 250. pEL3C18 - J23116 - CTX-M - lacIs Experiment: Restreak 60. pSB1A3 - B0032 - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Experiment: Assembly and transformation 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs Sunday 19 August Participant: Karl Experiment: Plasmid prep. 263. pGEM - eforRed 264. pGEM - aeBlue 265. pGEM - cjBlue Experiment: Cloning from PCR product 87. pSB1C3 - pBad/AraC Participant: Caroline, Katarina Experiment: Screening 60. pSB1A3 - B0032 - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Experiment: Plasmid prep. 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2 258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2 259. pEL4C15 - J23106 - CTX-M - linker - SYFP2 Experiment: Transformation 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs Experiment: O/N 60. pSB1A3 - B0032 - SYFP2 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR 250. pEL3C18 - J23116 - CTX-M - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Monday 20 August Participant: Caroline, Katarina Experiment: Plasmid prep. 60. pSB1A3 - B0032 - SYFP2 228. åEL3S17 - Plac - B0034 - SYFP2 - AmpR 250. pEL3C18 - J23116 - CTX-M - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Experiment: O/N 60. pSB1A3 - B0032 - SYFP2 Tuesday 21 August Participant: Anna, Fredrik Experiment: Assembly and transformation 204. pSB1K3 - B0034(mod) - marR(mut) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3T5 - J23110 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - marR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Experiment: O/N 152. pEL3C18 - J23106 - tetA - lacIs 153. pJN3C1 - Red 154. pSB1C3 - PT5lac 155. pSB1A2 - lacI 156. pJN3C1 - AmpR Participant: Karl Experiment: Assembly and transformation 266. pSB1C3 - eforRed 267. pSB1C3 - aeBlue 268. pSB1C3 - cjBlue Participant: Arvid Experiment: Mutagenesis pEL3K16 - redq (from red) pEL8(A-T)17(FRT) - redq Experiment: Mutagenesis pEL3K16iq - red -> redq pEL4[A-T]17(FRT) - red -> redq Result: No band on gel. Participant: Caroline, Katarina, Emy Experiment: Plasmid prep. 228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR Experiment: O/N 58. pSB3T5 - J23116 - CTX-M 114. pSB3T5 - J23110 - CTX-M 134. pSB1A3 - J23116 - tetA 189. pSB4K5 - lacIs Experiment: Digestion with notI 12. pSB1A2 - B0034 79. pSB1C3 - J23106 - B0034 Experiment: Streak from frozen stock 3. pSB1C3 - red 39. pSB1K3 - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 114. pSB3T5 - J23110 - CTX-M 132. pSB1C3 - Pxis 134. pSB1A3 - J23116 - tetA 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs 189. pSB4K5 - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Participant: Julia, Sabri Experiment: Transformation MG1655 225 + sRNA MG1655 257 + sRNA Result: Positive controll failed, has to be redone. Participant: Sabri, Julia Experiment: Making competent MG1655 cells containing reporter systems 225 (SYFP2) and 257 (lacIs) Experiment: Transforation of randomized sRNAs into previously made competent cells. Thursday 23 August Participant: Joakim Experiment: Screening 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16 - PT5lac B0034 Result: No correct band. Participant: Joel Experiment: Assembly 301. pSB3T5 - Plac - B0032 - SYFP2 302. pSB3T5 - PlaciQ - B0032 - SYFP2 303. pSB3T5 - PT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - Ptac - B0032 - SYFP2 310. pSB3T5 - Pxis - B0032 - SYFP2 Participant: Joel, Karl Experiment: Restreak 251. pEL3C18 - J23116 - tetA - lacIs 300. pEL3C18 - J23116 - CTX-M - linker - lacIs 301. pSB3T5 - Plac - B0032 - SYFP2 302. pSB3T5 - PlaciQ - B0032 - SYFP2 303. pSB3T5 - PT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - Ptac - B0032 - SYFP2 310. pSB3T5 - Pxis - B0032 - SYFP2 311. pSB3T5 - B0032 - SYFP2 316. pEL3A18 - J23110 - CTX-M - SYFP2 317. pEL3A18 - Plac - B0034 - SYFP2 - AmpR - T0 Participant: Caroline, Katarina Experiment: Assembly and transformation 248. pEL4C15 - J23110 - CTX-M - SYFP2 249. pEL4C15 - J23110 - tetA - SYFP2 250. pEL3C18 - J23116 - CTX-M - lacIs 251. pEL3C18 - J23116 - tetA - lacIs 311. pSB3T5 - B0032 - SYFP2 Experiment: Plasmid prep. 3. pSB3T5 - red 39. pSB1K3 - B0034 - SYFP2 58. pSB3T5 - J23116 - CTX-M 114. pSB3T5 - J23110 - CTX-M 132. pSB1C3 - Pxis 134. pSB1A3 - J23116 - tetA 189. pSB4K5 - lacIs Experiment: Mutagenesis 250. pEL3C18 - J23116 - CTX-M - lacIs Participant: Arvid Experiment: Mutagenesis pEL3K16iq - red -> redq pEL4[A-T]17(FRT) - red -> redq Participant: Anna, Fredrik Experiment: Screening and O/N 204. pSB1K3 - B0034(mod) - marR(mut) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3T5 - J23110 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - marR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Result: 204, 208 and 211 had good clones, 206, 207 and 261 did not. Participant: Julia Experiment: Assembly and transformation 164. pSB3T5 - Pxis - B0034 - SYFP2 Friday 24 August Participant: Pikkei, Karl Experiment: Cloning 266. pSB1C3 - eforRed 267. pSB1C3 - aeBlue 268. pSB1C3 - cjBlue 320. pSB1C3 - PT5Imm Participant: Hampus Experiment: Transformation MG1655 258 MG1655 259 Experiment: Restreak 164. pSB3T5 - Pxis - B0034 - SYFP2 Experiment: O/N MG1655 225 MG1655 257 Participant: Joakim Experiment: Screening 245. pEL3S17 - Plac - B0034 - SYFP2 262. pEL3K16iq - PT5lac - B0034 Result: All wrong. Participant: Caroline, Katarina Experiment: PCR purification 300. pEL3C18 - J23116 - CTX-M - linker - lacIs Experiment: Streak from frozen stock 60. pSB1A3 - B0032 - SYFP2 Experiment: Screening 12. pSB1A2 - B0034 79. pSB3T5 - J23106 - B0034 Experiment: Transforming 300. pEL3C18 - J23116 - CTX-M - linker - lacIs 251. pEL3C18 - J23116 - tetA - lacIs 311. pSB3T5 - B0032 - SYFP2 Participant: Joel Experiment: Assembly 316. pEL3A18 - J23110 - CTX-M - SYFP2 317. pEL3A18 - J23110 - tetA - SYFP2 Experiment: Transformation 301. pSB3T5 - Plac - B0032 - SYFP2 302. pSB3T5 - PlaciQ - B0032 - SYFP2 303. pSB3T5 - PT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - Ptac - B0032 - SYFP2 310. pSB3T5 - Pxis - B0032 - SYFP2 316. pEL3A18 - J23110 - CTX-M - SYFP2 317. pEL3A18 - J23110 - tetA - SYFP2 Saturday 25 August Participant: Hampus, Sabri Experiment: Screening 165. pSB3T5 - Pxis - B0034 - SYFP2 Experiment: Restreak 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0 320. pSB1C3 - PT5Imm Experiment: Transformation of sRNA into MG1655 containing 257 Participant: Fredrik Experiment: Assembly and transformation 202. pSB1K3 - B0034(mod) - LexA(G85D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3Y5 - J23110 - B0034 - OmpF(rsr) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Participant: Caroline, Katarina Experiment: Transformation 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 Participant: Lisa Experiment: Restreak MG1655 258 pEL4C15 - PlacIQ - CTX-M - linker - SYFP2 MG1655 259 pEL4C15 - J23106 - CTX-M - linker - SYFP2 Sunday 26 August Participant: Joel Experiment: Screening 301. pSB3T5 - Plac - B0032 - SYFP2 302. pSB3T5 - PlaciQ - B0032 - SYFP2 303. pSB3T5 - PT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - Ptac - B0032 - SYFP2 310. pSB3T5 - Pxis - B0032 - SYFP2 311. pSB3T5 - B0032 - SYFP2 Result: Empty, new gel tomorrow. Participant: Caroline, Katarina Experiment: Transformation 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 with 92. sRNA Experiment: Restreak 202. pSB1K3 - B0034(mod) - LexA(G85D) 203. pSB1K3 - B0034(mod) - OmpF(rsr) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Experiment: Screening 251. pEL3C18 - J23116 - tetA - lacIs 300. pEL3C18 - J23116 - CTX-M - linker - lacIs 316. pEL3A18 - J23110 - CTX-M - SYFP2 317. pEL3A18 - J23110 - tetA - SYFP2 Result: All but 251 had good clones. Experiment: O/N 300. pEL3C18 - J23116 - CTX-M - linker - lacIs 316. pEL3A18 - J23110 - CTX-M - SYFP2 317. pEL3A18 - J23110 - tetA - SYFP2 Experiment: Phusion PCR 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2 228. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2 259. pEL4C15 - J23106 - CTX-M - linker - SYFP2 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Participant: Hampus Experiment: Transformation sRNA Experiment: Restreak 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3Y5 - J23110 - B0034 - OmpF(rsr) Experiment: Assembly 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - T0 318 - pEL3S16 - Plac - B0034 - SYFP2 - AmpR - T0 Monday 27 August Participant: Joel, Fredrik Experiment: Screening 301. pSB3T5 - Plac - B0032 - SYFP2 302. pSB3T5 - PlaciQ - B0032 - SYFP2 303. pSB3T5 - PT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - Ptac - B0032 - SYFP2 310. pSB3T5 - Pxis - B0032 - SYFP2 311. pSB3T5 - B0032 - SYFP2 Result: All had good clones. Participant: Fredrik Experiment: Restreak 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0 Participant: Joakim Experiment: O/N 256. N3T Participant: Julia, Hampus Experiment: Plasmid prep. 164. pSB3T5 - Pxis - B0034 - SYFP2 Tuesday 28 August Participant: Hampus, Julia Experiment: Screening 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Result: Good. Experiment: Restreak 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Experiment: O/N 164. pSB3T5 - Pxis - B0034 - SYFP2 Participant: Sabri Experiment: Transformation 225+92 (reporter system + sRNA) 257+92 (reporter system + sRNA) 225+79 (to measure fitness cost for carrying two plasmids) 257+79 (to measure fitness cost) Participant: Olle Experiment: Screening 87. pSB1C3 - pBad Result: All bands wrong. Participant: Joel, Karl Experiment: Mutagenesis, screening and PCR purification 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Screening: Gel looked OK, but the concentration after the PCR purification gave negative results. Participant: Joakim Experiment: Screening 202. pSB1K3 - B0034(mod) - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Result: No good bands. Experiment: Viability test of 256. MG1655/N3T Wednesday 29 August Participant: Joel Experiment: Screening 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs 320. pSB1C3 - pT5Imm Result: Gel showed no bands. Participant: Joakim Experiment: Transformation M13mp18 Experiment: O/N MG1655 - pSa MG1655 - R1drd19 MG1655 (cipro resistant) Experiment: Counting colonies for the conjugation test Thursday 30 August Participant: Fredrik Experiment: Screening and O/N 202. pSB1K3 - B0034(mod) - LexA(G85D) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Participant: Joel, Karl, Julia, Hampus Experiment: Screening and O/N 305. pSB3T5 - J23101 - B0032 - SYFP2 Experiment: Mutagenesis 178. pSB3C18 - J23116 - AAC(6') - linker - lacIs 179. pSB3C18 - PlacIQ - AAC(6')- linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Experiment: Digestion with notI and screening 302. pSB3T5 - placiQ - B0032 - SYFP2 303. pSB3T5 - pT5lac - B0032 - SYFP2 304. pSB3T5 - PLlacO - B0032 - SYFP2 305. pSB3T5 - J23101 - B0032 - SYFP2 306. pSB3T5 - J23106 - B0032 - SYFP2 307. pSB3T5 - J23110 - B0032 - SYFP2 308. pSB3T5 - J23116 - B0032 - SYFP2 309. pSB3T5 - ptac - B0032 - SYFP2 310. pSB3T5 - pxis - B0032 - SYFP2 Result: Bad gel. Participant: Julia, Hampus Experiment: PCR purification RFP Experiment: Plasmid prep. 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Joakim Experiment: Conjugation test on 252+253 and 252+255 Friday 31 August Participant: Joel Experiment: Plasmid prep. 305. pSB3T5 - J23101 - B0032 - SYFP2 Experiment: Mutagenesis 317. pEL3A18 - J23110 - tetA - SYFP2 Experiment: PCR purification 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker - lacIs Participant: Hampus Experiment: Cloning to 1C3 J23101 - Spot42 J23106 - AAC(6') - linker - SYFP2 Plac - B0034 - SYFP2 PT5lac - B0034 J23116 - AAC(6') - linker - lacIs Participant: Johan Experiment: Mutagenesis pSB2K3 - tet inv. (H64A) pSB2K3 - tet inv. (E147A) pSB2K3 - tet inv. (H100A) pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (H64A) pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (E147A) pSB3T5 - J23110 - tet inv. B0034 - SYFP2(H100A) Participant: Julia Experiment: Assembly 325. pSB1C3 - J23101 - Spot42 326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2 327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Joakim, Fredrik Experiment: Preparation of competent cells DA25015 DA3006 Saturday 1 September Participant: Julia, Hampus Experiment: Lambda red PCR 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Sunday 2 September Participant: Hampus Experiment: Screening 262. pEL3K16 - PT5lac - B0034 325. pSB1C3 - Plac - B0034 - SYFP2 326. pEL3K16 - PT5lac - B0034 327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0 Monday 3 September Participant: Sabri Experiment: Screening 262. pEL3K16 - PT5lac - B0034 325. pSB1C3 - J23101 - Spot42 326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2 327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Caroline, Katarina, Joel Experiment: PCR purification, DpnI treatment, PCR purification 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Participant: Assembly 331. pEL3A18 - J23110 - tetA - linker - SYFP2 Participant: Hampus Experiment: Isolation of non-fluorescent sRNA Tuesday 4 September Participant: Caroline, Katarina, Joel, Emy Experiment: Assembly and transformation 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 Experiment: Transformation 331. pEL3A18 - J23110 - tetA - linker - SYFP2 Experiment: Lambda red into DA24100 and DA26250 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Hampus Experiment: Lambda red PCR 180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2 213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2 Wednesday 5 September Participant: Olle Experiment: O/N 349. pJNEG04 - miniF - SYFP2 - CTX-M Participant: Anton Experiment: Screening 332. pEL4T15 - PLlacO - B0034 - TAL2 334. pSB3K3 - PT5lac - B0034 - TAL1 Experiment: Restreak 346. pSB1K3 - B0034 - TAL2 Participant: Hampus Experiment: Transformation, plasmid prep. sRNA Spot42 Participant: Joel, Anna, Karl Experiment: Transformation 280. pEL4A15Iq - pUC - red 281. pEL4C15Iq - pUC - red 283. pEL4S15Iq - pUC - red 284. pEL4T15Iq - pUC - red 285. pEL4A15(FRT)Iq - pUC - red 286. pEL4C15(FRT)Iq - pUC - red 287. pEL4K15(FRT)Iq - pUC - red 288. pEL4S15(FRT)Iq - pUC - red Experiment: Restreak 331. pEL3A18 - J23110 - tetA - linker - SYFP2 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 Thursday 6 September Participant: Joel Experiment: Screening 331. pEL3A18 - J23110 - tetA - linker - SYFP2 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 Result: Screening failed. Experiment: Restreak 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Participant: Sabri, Lisa, Hampus Experiment: Transformation, plasmid prep 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2 Experiment: Restreak non-fluorescent sRNA clones Experiment: Mutagenesis 24. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Friday 7 September Participant: Hampus Experiment: Screening 326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2 327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0 348. pSB1C3 - J23116 - AAC(6') - linker - lacIs Participant: Hampus, Sabri Experiment: Transformation Randomized sRNA Experiment: Restreak Isolated sRNAs Experiment: O/N pSB1C3 - J23101 - Spot42 Participant: Joakim Experiment: Ligation 202. pSB1K3 - B0034(mod) - LexA(G85D) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3Y5 - J23110 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - MarR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Participant: Olle Experiment: Mutagenesis of red to redq on 280. pEL4A15Iq - pUC - red 281. pEL4C15Iq - pUC - red 283. pEL4S15Iq - pUC - red 285. pEL4A15(FRT)Iq - pUC - red 286. pEL4C15(FRT)Iq - pUC - red 287. pEL4K15(FRT)Iq - pUC - red Saturday 8 September Participant: Joel, Joakim Experiment: Screening 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 lac:amp Result: 193 showed only primer dimers, lac:amp had good clones. Participant: Lisa, Sabri Experiment: Transformation sRNAs into MG1655 sRNAs into 225 Experiment: Prepare cell cultures for cell sorting using FACS Participant: Joakim Experiment: Transformation 202. pSB1K3 - B0034(mod) - LexA(G85D) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3Y5 - J23110 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - MarR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Experiment: Screening 178. pEL3C18 - J23116 - AAC(6') - linker - lacIs 179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs 219. pEL3C18 - J23106 - tetA - linker - lacIs 220. pEL3C18 - J23106 - AAC(6') - linker lacIs 221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs 222. pEL3C18 - PlacIQ - tetA - linker - lacIs 260. pEL3C18 - J23106 - CTX-M - linker - lacIs Result: No bands visible. Participant: Sabri Experiment: Screening 24. pSB2K3 - tet inv. 226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2 227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2 Sunday 9 September Participant: Olle Experiment: Screening pEL4-series TALENs Result: Backbones looked good, TALENs gave no bands. Monday 10 September Participant: Joel, Olle, Pikkei Experiment: Assembly and transformation 336. pSB3S5 - plac - B0032 - SYFP2 337. pSB3S5 - placiQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 357. pSB4C5 - J23110 - B0032 - SYFP2 358. pSB4K5 - J23110 - B0032 - SYFP2 359. pEL4T15 - J23110 - B0032 - SYFP2 360. pEL4C15 - J23110 - B0032 - SYFP2 Experiment: Lambda red PCR 193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0 Experiment: O/N lac:amp Participant: Anna, Fredrik Experiment: Screening and O/N 202. pSB1K3 - B0034(mod) - LexA(G85D) 206. pSB3T5 - J23101 - B0034 - OmpF(rsr) 207. pSB3Y5 - J23110 - B0034 - OmpF(rsr) 208. pSB3T5 - J23101 - B0034 - MarR(mut) 211. pSB3T5 - PLlacO - B0034 - LexA(G85D) 261. pSB3K3 - PT5lac - B0034 - LexA(G85D) Participant: Olle Experiment: Assembly and transformation 333. pEL3K16iq - J23110 - B0034 - eforRed 241. pEL4S17iq - red 243. pEL4S17iq - J23110 - B0034 - eforRed Tuesday 11 September Wednesday 12 September Participant: Olle, Karl, Anna, Joel Experiment: Screening 336. pSB3S5 - plac - B0032 - SYFP2 337. pSB3S5 - placiQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 357. pSB4C5 - J23110 - B0032 - SYFP2 358. pSB4K5 - J23110 - B0032 - SYFP2 359. pEL4T15 - J23110 - B0032 - SYFP2 360. pEL4C15 - J23110 - B0032 - SYFP2 Participant: Joakim Experiment: O/N MG1655 225 MG1655 257 sRNA Thursday 13 September Participant: Hampus Experiment: Screening 325. pSB1C3 - J23101 - Spot42 361. pSB1C3 - RFP - linker - SYFP2 362. pSB1C3 - J23116 - AAC(6') - linker - lacIs Result: 325 and 361 gave good bands, 362 gave no bands at all. Participant: Julia Experiment: Transformation sRNA Participant: Emy, Caroline, Katarina Experiment: Tranformation 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 357. pSB4C5 - J23110 - B0032 - SYFP2 358. pSB4K5 - J23110 - B0032 - SYFP2 359. pEL4T15 - J23110 - B0032 - SYFP2 360. pEL4C15 - J23110 - B0032 - SYFP2 Experiment: Restreak 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 360. pEL4C15 - J23110 - B0032 - SYFP2 Friday 14 September Participant: Katarina, Caroline, Joel, Emy Experiment: Mutagenesis 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 - PlacIQ – tetA - linker - SYFP2 218. pEL3A18 - J23106 - tetA - linker - SYFP2 347. pEL3C18 - J23116 - tetA - linker - lacIs Experiment: Screening and O/N 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 360. pEL4C15 - J23110 - B0032 - SYFP2 Participant: Julia Experiment: O/N MG1655 with 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 MG1655 with 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2 Experiment: Plasmid prep. 86. pSB1A2 - PLlacO 361. pSB1C3 - RFP - linker - SYFP2 362. pSB1C3 - J23116 - AAC(6') - linker - lacIs Saturday 15 September Participant: Julia Experiment: Restreak, plasmid prep. sRNAs Experiment: Screening, PCR purification and digestion with DpnI AC6 - Spot42 Friday 21 September Participant: Joel Experiment: Restreak 215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2 216. pEL3A18 PlacIQ – tetA - linker - SYFP2 218. pEL3A18 J23106 - tetA - linker - SYFP2 382. pSB4K5 - J23101 - B0032 - SYFP2 383. pEL4T15 - J23101 - B0032 - SYFP2 384. pEL8C15 - J23101 - B0032 - SYFP2 Experiment: Streak from frozen stock 336. pSB3S5 - Plac - B0032 - SYFP2 337. pSB3S5 - PlaciQ - B0032 - SYFP2 338. pSB3S5 - T5lac - B0032 - SYFP2 339. pSB3S5 - PLlacO - B0032 - SYFP2 340. pSB3S5 - J23101 - B0032 - SYFP2 341. pSB3S5 - J23106 - B0032 - SYFP2 342. pSB3S5 - J23110 - B0032 - SYFP2 343. pSB3S5 - J23116 - B0032 - SYFP2 344. pSB3S5 - Ptac - B0032 - SYFP2 345. pSB3S5 - Pxis - B0032 - SYFP2 |
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