Team:WashU/Protocols/SDSPAGE15
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SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel
To create a 15% resolving gel with a 4% stacking gel, use:
15% | |
---|---|
dH2O | 1.2 ml |
30% acrylamid/0.8% methylene bisacrylamide | 2.5 mls |
4x Lower Tris | 1.21875 ml |
10% Temed | .0025 ml |
20% SDS | .250 ml |
10% ammonium persulfate (APS) | .01 ml |
To make the 4% stacking gel, use:
4% | |
---|---|
dH2O | 1.845 ml |
30% acrylamid/0.8% methylene bisacrylamide | .402 mls |
4x Upper Tris | .75 ml |
10% Temed | .003 ml |
20% SDS | .015 ml |
10% ammonium persulfate (APS) | .006 ml |