SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel

To create a 15% resolving gel with a 4% stacking gel, use:

**Table 1:** Preparation of the separating gel solution for 1 gel
	15%
dH₂O	1.2 ml
30% acrylamid/0.8% methylene bisacrylamide	2.5 mls
4x Lower Tris	1.21875 ml
20% SDS	.250 ml
10% Temed	.0025 ml
25% ammonium persulfate (APS)	.01 ml

To make the 4% stacking gel, use:

**Table 1:** Preparation of the stacking gel solution for 1 gel
	4%
dH₂O	1.845 ml
30% acrylamid/0.8% methylene bisacrylamide	.402 mls
4x Upper Tris	.75 ml
20% SDS	.015 ml
10% Temed	.003 ml
25% ammonium persulfate (APS)	.006 ml

Team:WashU/Protocols/SDSPAGE15