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From 2012.igem.org

Home Team Official Team Profile Project Parts Submitted Modeling Notebook Attributions

Contents 1 Notebook for week of July 9 to July 15 1.1 Design of Converter Plasmid 1.2 Design of Reporter Plasmid 1.3 Spinach Aptamer 1.4 CBB5 Caffeine Inducible Promoters 1.5 Inducible Odor

Notebook for week of July 9 to July 15

Design of Converter Plasmid

• Razan
  • 7/9- Gibson Assembly of Converter and Control plasmids
  • 7/10- Transformation of gibson failed and gel shows no correct bands
  • 7/11- Redid Gibson assembly of Converter and Control plasmids with longer incubation time; same results.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

• Peter
  • 7/9 - Set up confirmation PCR of cultures. 1 for each culture, as well as 2 controls from CBB5 genomic DNA.
  • 7/10 - Gel electrophoresis of confirmation PCR showed 2 successful Gibson product colonies, as well as both successful controls from CBB5 genomic DNA.

Inducible Odor