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From 2012.igem.org

Week 24: 8 october - 14 october

GFP modification

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11 October

• grow JM109 containing our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter)and induce expression with IPTG

-> no conclusion could be made due to growth on the negative control

• send samples of BBa_K883702 BBa_K883703 BBa_K883701 and BBa_K883700 in for sequencing to check if the last transformation from pJET into the iGEM backbones succeded

-> sequencing results where never obtained - the samples did not arrive

12 October

• 2nd time growing JM109 containing our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter) and induce expression with IPTG

-> again there is growth on the negative control -> no green fluorescence could be seen

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