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Team:Wageningen UR/Journal/week24
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Week 24: 8 october - 14 october
GFP modification
11 October
- grow our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter) and induce expression with IPTG
-> no conclusion could be made due to growth on the negative control
- send samples of BBa_K883702 BBa_K883703 BBa_K883701 and BBa_K883700 in for sequencing to check if the last transformation from pJET into the iGEM backbones succeded
12 October
- 2nd time growing our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter) and induce expression with IPTG
-> again there is growth on the negative control -> no green fluorescence could be seen
Hepatitis B inside modification
12 October
- PCR check of the PCR fragment obtained on 27.August
Figure 1: The purified PCR fragment contains fragments of the correct size as well as a bigger fragment. The nonpurified sample shows only a smere.'
