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Team:Cornell/testing/project/wetlab/4/7
From 2012.igem.org
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Wet Lab
- Overview
- Chassis
- DNA Assembly
- Testing & Results
- Future Work
- Animation
| Name | Type | Description | Designer | Length | |||
|---|---|---|---|---|---|---|---|
| BBa_K957000 | Generator | Arsenic inducible mtrB with cut sites flanking RBS | Dylan Patrick Webster | 2625 | |||
| BBa_K957001 | Generator | Arsenic inducible mtrB | Dylan Patrick Webster | 2704 | |||
| BBa_K957002 | Generator | Salicylate inducible mtrB with cut sites flanking RBS | Dylan Patrick Webster | 3207 | |||
| BBa_K957003 | Generator | Salicylate inducible mtrB | Dylan Patrick Webster | 3284 | |||
| BBa_K957004 | Coding | nah Operon for degradation of naphthalene to salicylate | Dylan Patrick Webster | 9409 | |||
| BBa_K957005 | Generator | Constitutive expression of nah Operon for degradation of naphthalene to salicylate | Dylan Patrick Webster | 9452 | |||
| BBa_K957006 | Composite | Constitutive expression of nah Operon with oriT | Dylan Patrick Webster | 9831 |
First Lessons Learned: Control Reactors
In order to enhance the field-deployability of our final device, we initially decided to feed our reactors with LB, since a very concentrated LB source fed at a low flow rate could sustain our field reactors for extended periods of time without taking up much physical space. However, upon setting up control reactors—both in batch and continuous flow operation—we discovered that wild type S. oneidensis MR-1 produced significantly less current when fed with LB than M4—a commonly used media for Shewanella-inoculated bioelectrochemical systems.Naphthalene & Salicylate Sensing
First characterized naphthalene sensors with salicylate, since our system indirectly senses naphthalene via salicylate.Uninduced salicylate reporter produces same current as MR-1 in rich M4 media.
First attempt to diagnose problem: Switch to minimal M4... Producing higher current, but we're still not getting a salicylate response.
Next attempt: Poise the working electrode at a higher potential in attempt to get more MtrC and MtrA for MtrB to associate with. Didn't quite do the trick either.
