Team:Michigan/Notebook/Protocols

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Xayona Website Template

Protocols

General

Plate Production

Cloning

Removing BioBricks from the Registry

Production of Competent Cells

Heat Shock Transformation

DNA Purification

Restriction Enzyme Digest

Ligation

PCR (Phusion)

Colony PCR

Characterization

Transmission Electron Microscopy Protocol

FimE Asymmetrical Digest Assay Protocol

File:MichRem.pdf https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf File:MichElectro.pdf https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf File:MichHeat.pdf https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf File:MichLig.pdf https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf File:MichPCR.pdf https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf File:MichCol.pdf https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf File:MichPlate.pdf https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf File:MichTEM.pdf

File:MichAsm.pdf https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf

File:MichPur1.pdf https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf File:MichPur2.pdf https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf

File:MichRestrict.pdf https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf File:MichComp.pdf https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf