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Team:Michigan/Notebook/Protocols
From 2012.igem.org
Protocols
General
Plate Production[1]Cloning
Removing BioBricks from the Registry[2]Production of Competent Cells[3]
Heat Shock Transformation[4]
DNA Purification: PCR[5] DNA Purification: MiniPrep[6]
Restriction Enzyme Digest[7]
Ligation[8]
PCR (Phusion)[9]
Colony PCR[10]
Gel Electrophoresis[11]
Characterization
Transmission Electron Microscopy Protocol[12]
FimE Asymmetrical Digest Assay Protocol[13]
