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User:DrJones1935/10 September 2012
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I. Measure the Concentration of Miniprepped Plasmids
- Bring samples and Buffer EB bottle upstairs to Take3 plate reader
- Add 2 uL of each sample according to the following chart:
| Blank (EB) | 1:1 #1 |
| Blank | 1:1 #2 |
| Blank | 1:1 #3 |
| Blank | 1:1 #4 |
| Blank | 1:2 #1 |
| Blank | |
| Blank | |
| Blank |
- Use the program to blank the machine and measure sample concentrations
RESULTS
Concentration:
| Plasmid | Concentration (ng/uL) | Approx. volume (uL) | Approx. total (ug) |
|---|---|---|---|
| 1:1 #1 | 32.297 | ~42 | 1.36 |
| 1:1 #2 | 28.158 | ~42 | 1.18 |
| 1:1 #3 | 23.037 | ~42 | 0.97 |
| 1:1 #4 | 31.93 | ~42 | 1.34 |
| 1:2 #1 | 27.983 | ~42 | 1.18 |
- Store DNA at 4 oC
