User:DrJones1935/10 September 2012
From 2012.igem.org
I. Measure the Concentration of Miniprepped Plasmids
- Bring samples and Buffer EB bottle upstairs to Take3 plate reader
- Add 2 uL of each sample according to the following chart:
| Blank (EB) | 1:1 #1
|
| Blank | 1:1 #2
|
| Blank | 1:1 #3
|
| Blank | 1:1 #4
|
| Blank | 1:2 #1
|
| Blank |
|
| Blank |
|
| Blank |
|
- Use the program to blank the machine and measure sample concentrations
RESULTS
Concentration:
| Plasmid | Concentration (ng/uL) | Approx. volume (uL) | Approx. total (ug)
|
| 1:1 #1 | 32.297 | ~42 | 1.36
|
| 1:1 #2 | 28.158 | ~42 | 1.18
|
| 1:1 #3 | 23.037 | ~42 | 0.97
|
| 1:1 #4 | 31.93 | ~42 | 1.34
|
| 1:2 #1 | 27.983 | ~42 | 1.18
|
"
