From 2012.igem.org
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Serial Dilutions
- Grow overnight culture of Normal Origami cells, Normal Origami cells exposed to IPTG, Transformed Origami cells exposed to IPTG
-
- Prepare 24 1.5mL eppendorf tubes filled with 450uL of LB media (3 rows of 8 eppendorf tubes)
- Pipette 50uL of each cell culture into 3 of the eppendorf tubes prepared
- Mix the eppendorf tube thoroughly with a pipette tip
- Pipette 50uL of mixed solution from the eppendorf tubes and transfer into another eppendorf tube of LB media
- Continue process 8 times
"
