"

From 2012.igem.org

I. Measure the Concentration of Miniprepped Plasmids

• Bring samples and Buffer EB bottle upstairs to Take3 plate reader
• Add 2 uL of each sample according to the following chart:

Blank (EB)	1:1 #1
Blank	1:1 #2
Blank	1:1 #3
Blank	1:1 #4
Blank	1:2 #1
Blank
Blank
Blank

• Use the program to blank the machine and measure sample concentrations

RESULTS

File: Media:srk_plasmid_10sep12.xlsx

Concentration:

Plasmid	Concentration (ng/uL)	Approx. volume (uL)	Approx. total (ug)
1:1 #1	32.297	~42	1.36
1:1 #2	28.158	~42	1.18
1:1 #3	23.037	~42	0.97
1:1 #4	31.93	~42	1.34
1:2 #1	27.983	~42	1.18

• Store DNA at 4 ^oC