User:DrJones1935/26 June 2012
From 2012.igem.org
I. Inoculate Liquid Cultures for Cultures Which Failed to Grow
- Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the following table in sterile culture tubes
Strain | Media |
---|---|
E. coli ECNR1 | LB |
E. coli SIMD43 | LB |
- Incubate cultures overnight with shaking at 37 oC