User:DrJones1935/22 June 2012

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I: Make 15% Glycerol Stocks (15 total) from Successful Overnight Cultures

  • Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3 per culture)
  • SIMD40
  • SIMD42
  • SIMD45
  • SIMD50
  • SIMD57
  • Add 200 uL of 60% glycerol to each tube (x3 per culture)
  • Vortex to mix thoroughly
  • Store tubes in the -80 oC freezer
  • Discard any remaining liquid culture from above cell lines

Note: The ECNR1 and SIMD43 strains have still not grown. Liquid cultures will be left over the weekend.

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