To develop strains of bacteria that produce high levels of 1-butanol we have introduced the genes coding for a biochemical pathway from Clostridium acetobutylicum into a mutant E. coli strain that produces a high level of NADH.The combination of these chemical pathways is predicted to increase the level of butanol production.. >>
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The Etch-a-Sketch project aims to create a lawn of bacteria that can be drawn on with a laser pointer. This seemingly inconsequential task actually presents many interesting engineering challenges. We have designed a novel genetic switch to tackle these problems. If our work proves successful, it will serve as a useful model for future projects that require large signal amplification.
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1. Would any of your project ideas raise safety issues in terms of?
Researcher Safety
Use of hazardous chemicals: Ethidium Bromide (EtBr)
Public Safety
None
Environmental Safety?
None
Security Threats?
None
Our projects are involved with Butanol pathways and light detection. By themselves they pose no risk to anyone. Although the yield of 1-Butanol must be handled with caution due to its flammability.
2. Do any of the new biobrick parts (or devices) that you made this year raise any safety issues? If yes,
For the parts that are already in the registry no safety issues have been encountered. The five BioBrick parts we are creating from scratch are transcriptional regulators and genes which should not raise any safety issues