![](https://static.igem.org/mediawiki/2012/f/f6/Plasmid3pBBR1.PNG)
pBBR1-aceEF.lpd – aceEF-lpd operon
Organism: DH10B
Operon: aceEF-lpd
http://www.biocyc.org/ECOL316385/NEW-IMAGE?type=OPERON&object=TUH9B-53
aceE aceF
The aceE coding region of DNA codes for a protein that operates as a pyruvate dyhydrogenase. This plays a key role in the beginning of the pathway, as the pyruvate is broken down into Acetyl-CoA. The aceF coding region of DNA codes for a protein that acts as a dihydrolipoyl transacetylase, which is also responsible for pyruvate decarboxylation which links glycolysis to the citric acid cycle.
aceE
(pyruvate dehydrogenase subunit)
Organism: DH10B
Amino Acid: 887
Nucleotides: 2664
http://www.genome.jp/dbget-bin/www_bget?ecd:ECDH10B_0094
Note: this gene has EcoRI site, needs to be optimized
aceF
(dihydrollpoamide acetyltransferase)
Amino Acids: 630
Nucleotides: 1893
http://www.genome.jp/dbget-bin/www_bget?ecd:ECDH10B_0095
lpd
(dihydrollpoamide dehydrogenase)
Amino Acids: 474
Nucleotides: 1425
http://www.genome.jp/dbget-bin/www_bget?ecd:ECDH10B_0096 |