Team:Missouri Miners

From 2012.igem.org

Revision as of 16:32, 30 September 2012 by Davrpoh (Talk | contribs)

Missouri Miners 2012

Adjustable Multi-Enzyme to Cell Surface Anchoring Protein

There are a plethora of enzymes that occur in the natural world which perform reactions that could be immensely useful to humans. Unfortunately, the efficiency of some of these reactions may render their applications logistically unrealistic. The cellulosome scaffolding protein produced by Clostridium thermocellum has been shown to significantly increase the efficiency of cellulose degradation. The scaffolding protein can be reduced in size and adapted for the cell surface of Escherichia coli. Different cohesion sites on the new cell surface display protein can also be introduced to allow for attachment of desired enzymes. Future applications would include producing a collection of distinct versions of the scaffolding protein for unique arrangements and concentrations of enzymes, enabling construction of an extra-cellular assembly line for a variety of multi-enzymatic reactions. This would lay the foundation for making previously infeasible applications of reactions possible through increased efficiency.

Cohesin Complex 22. Adams JJ, Currie MA, Ali S, Bayer EA, Jia Z, and Smith SP. Insights into higher-order organization of the cellulosome revealed by a dissect-and-build approach: crystal structure of interacting Clostridium thermocellum multimodular components. J Mol Biol 2010 Mar 5; 396(4) 833-9. doi:10.1016/j.jmb.2010.01.015 pmid:20070943. PubMed HubMed [cellulosome7]

Team History:


We are the Missouri Miners from Missouri University of Science and Technology. Our team formed in 2007 with just a handful of people working on iGEM projects. Now our team has grown to nearly 30 members working hard and learning new things in the lab.
Our team is a recognized student organization at S&T and just recently became a member of the Student Design Team, which is a highly respected tradition here on our campus. Our most recent accomplishment is forming a basic lab training program to get our new members more familiar in the lab and ready to work on future projects.

Retrieved from "http://2012.igem.org/Team:Missouri_Miners"