From 2012.igem.org

Preparation of Overnight Cultures

1. Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube.
2. Add ~ 3-5 mLs of an appropriate broth (either LB or TB)
3. Add an appropriate antibiotic if necessary and set this tube safely aside
   • Amp (1:100)
   • Chlor (1:1000)
   • Kan (1:1000)
4. Prick the desired colony off a plate and suspend it in 50 uL PBS.
   • Refer to the PBS stock making protocol!
5. Add 10 uL of the PBS sample and add it to the broth previously set aside.
   • Incubate overnight cultures @ 37oC, overnight with shaking.