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User contributions
From 2012.igem.org
- 03:47, 1 October 2012 (diff | hist) N Team:Washington/Protocols/Gel Purification (Created page with "=Gel Purification= #Using the QIAquick kit, first excise the DNA fragment form the agarose gel with a clean, sharp blade. #Weigh the gel slice in a tared colorless tube. Add 3 v...") (top)
- 03:29, 1 October 2012 (diff | hist) Team:Washington/Protocols
- 03:26, 1 October 2012 (diff | hist) N Team:Washington/Protocols/PCR Purification (Created page with "=PCR Purification= #Using the QIAquick kit, add 5 volumes of Buffer PB to 1 volume of the PCR sampe and mix. It is not necessary to remove mineral oil or kerosene. #If pH indica...")
- 03:16, 1 October 2012 (diff | hist) Team:Washington/Protocols
- 03:07, 1 October 2012 (diff | hist) Team:Washington/Protocols/osmY Assay
- 03:04, 1 October 2012 (diff | hist) N Team:Washington/Protocols/osmY Assay (Created page with "=osmY Assay= #Make M-9 media #Make 4 liquid culture tubes each of sfGFP-osmY (n-c), osmY-GFP (Bright) (c-n), iGEM2011-003 sfGFP-mamI 1A3, iGEM2011-009 plac Bright 1A3, and no ce...")
- 02:30, 1 October 2012 (diff | hist) Team:Washington/Protocols/gene assembly (→Gene Synthesis) (top)
- 02:18, 1 October 2012 (diff | hist) Team:Washington/Protocols
- 06:22, 29 September 2012 (diff | hist) N Team:Washington/Protocols/Overnights. (Created page with "{{Template:Team:Washington/Templates/Top}} =Preparation of Overnight Cultures= # Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube. # Add ~ 3-5 mLs of an appropriate brot...") (top)
- 06:21, 29 September 2012 (diff | hist) Team:Washington/Protocols
- 06:14, 29 September 2012 (diff | hist) N Team:Washington/Protocols/Plas DNA. (Created page with "{{Template:Team:Washington/Templates/Top}} =Isolation of Plasmid DNA (miniprep)= # Gently vortex overnight culture(s) to mix cells. # Pipet ~1 mL of cells into a labeled micro...")
- 06:13, 29 September 2012 (diff | hist) Team:Washington/Protocols
- 21:57, 19 September 2012 (diff | hist) Team:Washington/Plastics
- 21:55, 19 September 2012 (diff | hist) Team:Washington/Plastics
