Team/CINVESTAV-IPN-UNAM MX/Construction.htm

CHARACTERIZATION OF IGEM BIOPARTS DISTRIBUTION

As part of the points required to perform well in 2012 iGEM competition we are asked to characterize some existing biopartes on the record, so we decided to make the characterization of constitutive promoters belonging to the family isolated from a small combinatorial library (J23101 , J23102, J23104, J23107, J23108, J2311, and J23115) which were attached to GFP to determine promoter activity, using the equipment Victor X3 Multilabel Plate Reader.

Fig 1. General scheme of one construction of the promoter expressing GFP

Fig 2. Final construction