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Team:TU-Eindhoven/LEC/Lab
From 2012.igem.org
GECO protein expression and isolation
Plasmid construction
The following non-BioBrick plasmids have been constructed:
| name | vector | inserts | description |
|---|---|---|---|
| pYES2/CT-X-GECO | pYES2/CT | R-GECO1, G-GECO1.1, B-GECO1 | High copy-number shuttle vector |
| pYES3/CT-X-GECO | pYES3/CT | R-GECO1, G-GECO1.1, B-GECO1 | High copy-number shuttle vector |
| pET28a | X-GECO/pET28a | R-GECO1, G-GECO1.1, B-GECO1 | Expression vector for E. coli |
The following plasmids were received from addgene.org:
| name | vector | inserts | description |
|---|---|---|---|
| CMV-X-GECO | CMV | R-GECO1, G-GECO1.1, B-GECO1 | Shipping vector use by addgene.org, CMV vector was not used in this project |
These plasmids were kindly donated by H. Iida & K. Iida:
| name | vector | inserts | description |
|---|---|---|---|
| pBCT -CCH1H | pBCT | CCH1H | Low copy-number shuttle vector, encodes the CCH1 protein |
| pBCT-CCH1H-HA4 | pBCT | CCH1H-HA4 | Low copy-number shuttle vector, encodes HA4-tagged CCH1 |
| pBCT-CCH1H-EGFP | pBCT | CCH1H-EGFP | Low copy-number shuttle vector, encodes EGFP-tagged CCH1 |
| YCpT-MID1 | YCpT | MID1 | Low copy-number shuttle vector, encodes the MID1 protein |
| YCpT-MID1-EGFP | YCpT | MID1-EGFP | Low copy-number shuttle vector, encodes EGFP-tagged MID1 |
References
