2012 iGEM

CBNU-Korea

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1. BioBrick Database

- The program will be installed and run in the local computer. When the program is distributed to the user. If the whole information about BioBrick is included in the install program, this will not only increase the size of the install file, but also the user won’t need all of these informations. So we made our program allow the users to choose the BioBrick information that they want, and download it selectively.

- To bring the already registered BioBrick data, our program parse and save the simple informations(ID, Name, Description, Type) that is registered in http://partsregistry.org/fasta/parts/All_Parts.

- All this saved information, will be shown in the download screen for the user. By choosing the BioBrick that the user wants, and pressing the download button, the Bricks information (Suparts, Sequence, Status, Rating etc.) will be saved in the local database after parsed by XML Part Information (http://partsregistry.org/ xml/part.+BioBrick Name).

2. The user's BioBrick

- If the user wants to use a BioBrick with their own style, not the BioBrick that is already registered in the program, they can personally add a new BioBrick and design it.

3. Management of Sequence

3-1. Suffix, Prefix, Scar

- The program automatically process the restriction enzyme between the Bricks when the user designs the BioBrick. By pressing the ‘Scar’ icon and including the BioBrick in there, the program will analyze the sequences of the Bricks and automatically choose where to make the adequate Scar form.

3-2. Cloning

- When the user selects the Plasmid Backbone, the program will check the sequence and find the restriction enzyme site. If the Plasmid Backbone does not include in the restriction site of Prefix, Suffix, then the user has to choose another plasmid backbone.

- When the user choose one restriction site from each of Prefix, Suffix and press the ‘OK’ button. it will process the designed BioBrick and the Plasmid Backbone sequence and show it on the screen.

4. Screen Shot function

- The teams participating the iGEM with experiments should register first in BioBrick. In this process, the informations about BioBricks in Wiki Pages and documents can be used through graphic work.We hope that our program will lighten the load used in those kind of progress, so the users can be more focused on the experiment. This program is made to design and save the images at the same time.

5. BioBrick Data Export

- Our program can save the BioBrick that the user designed in various forms. The user can use the data right away without any additional work.

5-1. SBOL

- SBOL(Synthetic Biology Open Language) is the exchange standard for the genetic tools, the device modules, and the explanation of the system data. We use libSBOLc(SBOL C library) to save the designed information as a SBOL form, so that itcan be easily transferred to the user.

5-2. Genbank

- This program is designed to save the information as Genbank form, and we referred the form from http://www.ncbi.nlm.nih.gov/Sitemap/samplerecord.

5-3. Fasta

- The Fasta file was made to be saved as seen in below.

> Part_Name Type "Description"
Sequence