Team:Exeter/lab book/gibs/wk6

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ExiGEM2012 Lab Book Gibs wk6


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 27th - 31st August - 3rd - 7th September - Results: Part 1 - Results: Part 2
	Operon Construction: 13th - 17th August 2012 Tuesday 14.8.12 Transformation of wcfA-term (BBa_K764010) Wednesday 15.8.12 Transferred wcfA-term colonies to LB(tet) medium Thursday 16.8.12 wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C Supernatant discarded Fermentas protocol *Step 10* 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation Nanodropped for following results: (A) 329.7 ng/µl (B) 50.0 ng/µl (C) 206.6 ng/µl Made streak plates from culture dregs

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