Team:Exeter/lab book/gibs/wk6

Operon Construction  |  Glycobase

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**Wednesday 15.8.12**

Transferred wcfA-term colonies to LB(tet) medium

**Thursday 16.8.12**

wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C

Supernatant discarded

Fermentas protocol

Step 10 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation

Nanodropped for following results:

(A) 329.7 ng/µl

(B) 50.0 ng/µl

(C) 206.6 ng/µl

Made streak plates from culture dregs