"

From 2012.igem.org

Protocols  |  Single Gene Plasmids and Enzyme Characterisation  |  Showcasing Polysaccharide Production  |  The 3-Gene Inducible Plasmid Operon Construction  |  Glycobase

Protocols

Transformation Protocol from Invitrogen, for Top10 Competent Cells Transferring Cultures to Liquid Medium Mini Prep New England BioLabs Biobrick Assembly Protocol Annealing Oligomer Primers from Bioneer Biobrick Extraction Two-Step PCR Bacterial Genomic DNA Extraction Using GenElute PCR purification using QIAquick Linearized Plasmid Backbone Digestion and Ligation Protocol Preparation of IDTE Buffer and IDT Re-Suspension 3-Step PCR SDS-PAGE Protocol