Team:Exeter/lab book/proto

From 2012.igem.org

Protocol Home

Protocols
  • Transformation Protocol from Invitrogen, for Top10 Competent Cells
  • Transferring Cultures to Liquid Medium
  • Mini Prep
  • New England BioLabs Biobrick Assembly Protocol
  • Annealing Oligomer Primers from Bioneer
  • Biobrick Extraction
  • Two-Step PCR
  • Bacterial Genomic DNA Extraction Using GenElute
  • PCR purification using QIAquick
  • Linearized Plasmid Backbone Digestion and Ligation Protocol
  • Preparation of IDTE Buffer and IDT Re-Suspension
  • 3-Step PCR
  • SDS-PAGE Protocol
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